Linking terpene synthases to sesquiterpene metabolism in grapevine flowers

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dc.contributor.authorSmit, Samuel Jacobusen_ZA
dc.contributor.authorVivier, Melane Aletheaen_ZA
dc.contributor.authorYoung, Philip Richarden_ZA
dc.date.accessioned2019-03-22T12:44:12Z
dc.date.available2019-03-22T12:44:12Z
dc.date.issued2019
dc.descriptionCITATION: Smit, S. J., Vivier, M. A. & Young, P. R. 2019. Linking terpene synthases to sesquiterpene metabolism in grapevine flowers. Frontiers in Plant Science, 10:177, doi:10.3389/fpls.2019.00177.
dc.descriptionThe original publication is available at https://www.frontiersin.org
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstractGrapevine (Vitis vinifera L.) terpene synthases (VviTPS) are responsible for the biosynthesis of terpenic volatiles. Volatile profiling of nine commercial wine cultivars showed unique cultivar-specific variation in volatile terpenes emitted from grapevine flowers. The flower chemotypes of three divergent cultivars, Muscat of Alexandria, Sauvignon Blanc and Shiraz were subsequently investigated at two flower developmental stages (EL-18 and -26). The cultivars displayed unique flower sesquiterpene compositions that changed during flower organogenesis and the profiles were dominated by either (E)-β-farnesene, (E,E)-α-farnesene or (+)-valencene. In silico remapping of microarray probes to VviTPS gene models allowed for a meta-analysis of VviTPS expression patterns in the grape gene atlas to identify genes that could regulate terpene biosynthesis in flowers. Selected sesquiterpene synthase genes were isolated and functionally characterized in three cultivars. Genotypic differences that could be linked to the function of a targeted gene model resulted in the isolation of a novel and cultivar-specific single product sesquiterpene synthase from Muscat of Alexandria flowers (VvivMATPS10), synthesizing (E)-β-farnesene as its major volatile. Furthermore, we identified structural variations (SNPs, InDels and splice variations) in the characterized VviTPS genes that potentially impact enzyme function and/or volatile sesquiterpene production in a cultivar-specific manner.en_ZA
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fpls.2019.00177/full
dc.description.versionPublisher's version
dc.format.extent18 pages
dc.identifier.citationSmit, S. J., Vivier, M. A. & Young, P. R. 2019. Linking terpene synthases to sesquiterpene metabolism in grapevine flowers. Frontiers in Plant Science, 10:177, doi:10.3389/fpls.2019.00177
dc.identifier.issn1664-462X (online)
dc.identifier.otherdoi:10.3389/fpls.2019.00177
dc.identifier.urihttp://hdl.handle.net/10019.1/105553
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers Media
dc.rights.holderAuthors retain copyright
dc.subjectSesquiterpenesen_ZA
dc.subjectTerpenesen_ZA
dc.subjectGrapesen_ZA
dc.titleLinking terpene synthases to sesquiterpene metabolism in grapevine flowersen_ZA
dc.typeArticleen_ZA
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