Pathogenicity associated genes in Fusarium oxysporum f. sp. cubense race 4

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dc.contributor.authorSutherland, Reneen_ZA
dc.contributor.authorViljoen, Altusen_ZA
dc.contributor.authorMyburg, Alexander A.en_ZA
dc.contributor.authorVan den Berg, Noelanien_ZA
dc.date.accessioned2014-07-07T12:34:50Z
dc.date.available2014-07-07T12:34:50Z
dc.date.issued2013
dc.descriptionCITATION: Sutherland, R., Viljoen, A., Myburg, A. A. & Van den Berg, N. 2013. Pathogenicity associated genes in Fusarium oxysporum f. sp. cubense race 4. South African Journal of Science, 109(5/6), Art. #0023, doi: 10.1590/sajs.2013/20120023.en_ZA
dc.descriptionThe original publication is available at http://sajs.co.zaen_ZA
dc.description.abstractFusarium oxysporum f. sp. cubense (Foc) is a fungus that infects banana roots and causes a destructive plant disease called Fusarium wilt. Foc consists of three pathogenic races (Foc races 1, 2 and 4), classified according to their selective impairment of banana cultivars. Foc race 4 is economically important as it comprises strains that infect Cavendish bananas, which are the most widely planted variety of bananas in the world, in both the tropics (Foc TR4) and subtropics (Foc STR4). The aim of this study was to investigate which genes are potentially involved in fungal pathogenicity by comparing transcript-derived cDNA fragments (TDFs) from Foc STR4 and TR4 to those from non-pathogenic F. oxysporum using cDNA-AFLP analysis. This comparison resulted in the identification of 229 unique gene fragments which include the putative pathogenicity-related TDFs encoding chitinase class V (chsV), GTPase activating protein, Major Facilitator Superfamily (MFS) multidrug transporter and serine/threonine protein kinase (ste12) genes. Quantitative analysis of transcript abundance showed a significant increase in expression of chsV, MFS multidrug transporter and ste12 genes in Foc STR4 and TR4 compared with the non-pathogenic F. oxysporum. These genes play a role in escaping host defence responses and in cell wall degradation. In addition, pathogenicity-related genes from other formae speciales of F. oxysporum, such as the sucrose non-fermenting, cytochrome P450 and F-box protein required for pathogenicity genes, were significantly up-regulated in Foc STR4 and TR4 but not in F. oxysporum isolates non-pathogenic to banana. This study provides the first in vitro comparative analysis of TDFs expressed in pathogenic Foc race 4 isolates and non-pathogenic F. oxysporum isolates from banana.en_ZA
dc.description.urihttp://sajs.co.za/pathogenicity-associated-genes-fusarium-oxysporum-f-sp-cubense-race-4/ren%C3%A9-sutherland-altus-viljoen-alexander-myburg-no%C3%ABlani-van-den-bergen_ZA
dc.description.versionPublisher's versionen_ZA
dc.format.extent10 pages : illustrations (some color)en_ZA
dc.identifier.citationSouth African Journal of Scienceen_ZA
dc.identifier.citationSutherland, R., Viljoen, A., Myburg, A. A. & Van den Berg, N. 2013. Pathogenicity associated genes in Fusarium oxysporum f. sp. cubense race 4. South African Journal of Science, 109(5/6), Art. #0023, doi: 10.1590/sajs.2013/20120023.en_ZA
dc.identifier.issn1996-7489 (online)
dc.identifier.otherdoi: 10.1590/sajs.2013/20120023
dc.identifier.urihttp://hdl.handle.net/10019.1/95135
dc.language.isoen_ZAen_ZA
dc.publisherAcademy of Science of South Africaen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectBananas -- Diseases and pestsen_ZA
dc.subjectcDNA-AFLPen_ZA
dc.subjectFusarium oxysporum -- Fungalen_ZA
dc.subjectPathogenicityen_ZA
dc.subjectVirulenceen_ZA
dc.titlePathogenicity associated genes in Fusarium oxysporum f. sp. cubense race 4en_ZA
dc.typeArticleen_ZA
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