Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae

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dc.contributor.authorGovender, P.en_ZA
dc.contributor.authorDomingo, J. L.en_ZA
dc.contributor.authorBester, M. C.en_ZA
dc.contributor.authorPretorius, I. S.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2011-05-15T15:56:31Z
dc.date.available2011-05-15T15:56:31Z
dc.date.issued2008
dc.description.abstractIn many industrial fermentation processes, the Saccharomyces cerevisiae yeast should ideally meet two partially conflicting demands. During fermentation, a high suspended yeast count is required to maintain a satisfactory rate of fermentation, while at completion, efficient settling is desired to enhance product clarification and recovery. In most fermentation industries, currently used starter cultures do not satisfy this ideal, probably because nonflocculent yeast strains were selected to avoid fermentation problems. In this paper, we assess molecular strategies to optimize the flocculation behavior of S. cerevisiae. For this purpose, the chromosomal copies of three dominant flocculation genes, FLO1, FLO5, and FLO11, of the haploid nonflocculent, noninvasive, and non-flor-forming S. cerevisiae FY23 strain were placed under the transcriptional control of the promoters of the ADH2 and HSP30 genes. All six promoter-gene combinations resulted in specific flocculation behaviors in terms of timing and intensity. The strategy resulted in stable expression patterns providing a platform for the direct comparison and assessment of the specific impact of the expression of individual dominant FLO genes with regard to cell wall characteristics, such as hydrophobicity, biofilm formation, and substrate adhesion properties. The data also clearly demonstrate that the flocculation behavior of yeast strains can be tightly controlled and fine-tuned to satisfy specific industrial requirements. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation74
dc.identifier.citation19
dc.identifier.issn992240
dc.identifier.other10.1128/AEM.00394-08
dc.identifier.urihttp://hdl.handle.net/10019.1/9897
dc.subjectBiochemical engineering
dc.subjectCell culture
dc.subjectClarification
dc.subjectEnhanced recovery
dc.subjectFlocculation
dc.subjectForming
dc.subjectGenes
dc.subjectStrain
dc.subjectYeast
dc.subjectAnd recoveries
dc.subjectBiofilm formations
dc.subjectCell walls
dc.subjectEfficient
dc.subjectGene combinations
dc.subjectIndustrial fermentations
dc.subjectIndustrial requirements
dc.subjectMolecular strategies
dc.subjectNoninvasive
dc.subjectS. cerevisiae
dc.subjectSaccharomyces cerevisiae
dc.subjectStable expressions
dc.subjectStarter cultures
dc.subjectSubstrate adhesions
dc.subjectTranscriptional controls
dc.subjectYeast strains
dc.subjectFermentation
dc.subjectheat shock protein 30
dc.subjectADH2 protein, S cerevisiae
dc.subjectalcohol dehydrogenase
dc.subjectFLO1 protein, S cerevisiae
dc.subjectFLO5 protein, S cerevisiae
dc.subjectglucose
dc.subjectHSP30 protein, S cerevisiae
dc.subjectmannose
dc.subjectmembrane protein
dc.subjectMUC1 protein, S cerevisiae
dc.subjectSaccharomyces cerevisiae protein
dc.subjectbiofilm
dc.subjectfermentation
dc.subjectflocculation
dc.subjectfood industry
dc.subjectgene expression
dc.subjecthydrophobicity
dc.subjectoptimization
dc.subjectyeast
dc.subjectarticle
dc.subjectbacterial gene
dc.subjectcontrolled study
dc.subjectflo1 gene
dc.subjectFLO11 gene
dc.subjectflo5 gene
dc.subjectflocculation
dc.subjectgene expression
dc.subjectgene sequence
dc.subjecthydrophobicity
dc.subjectnonhuman
dc.subjectSaccharomyces cerevisiae
dc.subjectbiofilm
dc.subjectbiosynthesis
dc.subjectcell wall
dc.subjectchemistry
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectmetabolism
dc.subjectmethodology
dc.subjectmicrobiology
dc.subjectphysiology
dc.subjectpromoter region
dc.subjectSaccharomyces cerevisiae
dc.subjectAlcohol Dehydrogenase
dc.subjectBiofilms
dc.subjectCell Wall
dc.subjectFlocculation
dc.subjectGene Expression Regulation, Fungal
dc.subjectGlucose
dc.subjectHSP30 Heat-Shock Proteins
dc.subjectHydrophobicity
dc.subjectIndustrial Microbiology
dc.subjectMannose
dc.subjectMembrane Proteins
dc.subjectPromoter Regions (Genetics)
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.titleControlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae
dc.typeArticle
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