Prevalence of readily detected amyloid blood clots in unclotted type 2 diabetes mellitus and COVID-19 plasma : a preliminary report

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Date
2020-11-17
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BMC (part of Springer Nature)
Abstract
Background: Type 2 Diabetes Mellitus (T2DM) is a well-known comorbidity to COVID-19 and coagulopathies are a common accompaniment to both T2DM and COVID-19. In addition, patients with COVID-19 are known to develop micro-clots within the lungs. The rapid detection of COVID-19 uses genotypic testing for the presence of SARS-Cov-2 virus in nasopharyngeal swabs, but it can have a poor sensitivity. A rapid, host-based physiological test that indicated clotting severity and the extent of clotting pathologies in the individual who was infected or not would be highly desirable. Methods: Platelet poor plasma (PPP) was collected and frozen. On the day of analysis, PPP samples were thawed and analysed. We show here that microclots can be detected in the native plasma of twenty COVID-19, as well as ten T2DM patients, without the addition of any clotting agent, and in particular that such clots are amyloid in nature as judged by a standard fluorogenic stain. Results were compared to ten healthy age-matched individuals. Results: In COVID-19 plasma these microclots are significantly increased when compared to the levels in T2DM. Conclusions: This fluorogenic test may provide a rapid and convenient test with 100% sensitivity (P < 0.0001) and is consistent with the recognition that the early detection and prevention of such clotting can have an important role in therapy.
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CITATION: Pretorius, E., et al.2020. Prevalence of readily detected amyloid blood clots in unclotted Type 2 Diabetes Mellitus and COVID-19 plasma : a preliminary report. Cardiovasc Diabetol, 19:193, doi:10.1186/s12933-020-01165-7.
The original publication is available at https://cardiab.biomedcentral.com
Keywords
COVID-19 (Disease), Type 2 diabetes mellitus -- Pathogenesis, Blood coagulation disorders, Amyloid
Citation
Pretorius, E., et al.2020. Prevalence of readily detected amyloid blood clots in unclotted Type 2 Diabetes Mellitus and COVID-19 plasma : a preliminary report. Cardiovasc Diabetol, 19:193, doi:10.1186/s12933-020-01165-7
URI
http://hdl.handle.net/10019.1/110384
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Research Articles (Physiological Sciences)