Mitochondrial oxidative phosphorylation in low-flow hypoxia: Role of free fatty acids

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dc.contributor.authorLochner A.
dc.contributor.authorKotze J.C.N.
dc.contributor.authorBenade A.J.S.
dc.contributor.authorGevers W.
dc.date.accessioned2011-05-15T15:59:44Z
dc.date.available2011-05-15T15:59:44Z
dc.date.issued1978
dc.description.abstractThe mechanism of mitochondrial damage was investigated in hypoxic hearts, perfused at low pressure without exogenous substrate, as a model of myocardial ischaemia. Mitochondrial and tissue free fatty acid (FFA) contents were determined in control hearts (perfused aerobically at a higher pressure, without exogenous substrate), and in the hypoxic hearts; the functional capacities of mitochondria isolated from the two types of tissue were also compared. Mitochondrial FFA contents were found to be evaluated, relative to the controls, after 20 min of low-flow hypoxic perfusion. However, mitochondrial FFA contents were not different in control and hypoxic hearts after 70 min of perfusion. Low-flow hypoxic perfusion for 70 min caused a significant elevation of tissue C16:0, C18:1 FFA fractions, while total FFA and triglyceride contents were also increased. Accumulation of FFA in whole tissue was accompanied by a depression in mitochondrial function. Thus, after 20 min, both tissue FFA contents and ADP/O and RCI values of mitochondria isolated from control and hypoxic hearts were not different, whereas after 70 min, tissue FFA levels were significantly elevated in hypoxic hearts, with an equally significant depression in the function of isolated mitochondria.
dc.description.versionArticle
dc.identifier.citationJournal of Molecular and Cellular Cardiology
dc.identifier.citation10
dc.identifier.citation9
dc.identifier.issn222828
dc.identifier.urihttp://hdl.handle.net/10019.1/11340
dc.subjectfatty acid
dc.subjectanimal experiment
dc.subjectheart
dc.subjectheart muscle ischemia
dc.subjectmitochondrion damage
dc.subjectoxidative phosphorylation
dc.subjectrat
dc.subjectAcyl Coenzyme A
dc.subjectAlbumins
dc.subjectAnimal
dc.subjectAnoxia
dc.subjectCoronary Disease
dc.subjectFatty Acids, Nonesterified
dc.subjectFemale
dc.subjectGlycerides
dc.subjectMitochondria, Heart
dc.subjectOxidative Phosphorylation
dc.subjectPerfusion
dc.subjectRats
dc.titleMitochondrial oxidative phosphorylation in low-flow hypoxia: Role of free fatty acids
dc.typeArticle
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