Identification of a functional TATA element in the STA2 glucoamylase gene promoter from Saccharomyces cerevisiae

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dc.contributor.authorVivier M.A.
dc.contributor.authorPretorius I.S.
dc.date.accessioned2011-05-15T16:01:29Z
dc.date.available2011-05-15T16:01:29Z
dc.date.issued1998
dc.description.abstractAccurate transcription by RNA polymerase II is usually dependent on the presence of a TATA element, and/or an initiator element, in the promoters of protein-encoding genes. The STA1-3 genes, encoding three glucoamylase isozymes (Sta1p, Sta2p and Sta3p, respectively) responsible for starch hydrolysis in the yeast Saccharomyces cerevisiae, have been shown to contain long and complex promoters with several regulatory regions. These promoters are also virtually identical to the yeast MUC1 gene promoter; this gene encodes a mucin-like protein and is evolutionary linked to, and transcriptionally co-regulated with, STA1-3. The STA1-3 genes contain two putative TATA sequences, one conforming to the typical TATA box sequence, TATAAA, and another with the sequence of TATAAT. Here we present a study into the functional relevance of these putative TATA sequences and their effects on the transcription of the STA2 gene (as a representative model of the STA1-3 multigene family) and, by analogy, the MUC1 gene. We show that the TATAAA motif is the functional TATA box for STA2 and influences transcript levels, transcript initiation sites, and glucoamylase activities.
dc.description.versionArticle
dc.identifier.citationCurrent Genetics
dc.identifier.citation33
dc.identifier.citation1
dc.identifier.issn1728083
dc.identifier.other10.1007/s002940050302
dc.identifier.urihttp://hdl.handle.net/10019.1/12002
dc.subjectglucan 1,4 alpha glucosidase
dc.subjectisoenzyme
dc.subjectmucin
dc.subjectrna polymerase ii
dc.subjectstarch
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectdeletion mutant
dc.subjectenzyme activity
dc.subjectfungal genetics
dc.subjectgene expression
dc.subjectgene mapping
dc.subjectgenetic transcription
dc.subjecthydrolysis
dc.subjectmultigene family
dc.subjectnonhuman
dc.subjectnorthern blotting
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectregulator gene
dc.subjectsaccharomyces cerevisiae
dc.subjecttata box
dc.subjecttranscription initiation
dc.subjectEnzyme Activation
dc.subjectGene Deletion
dc.subjectGlucan 1,4-alpha-Glucosidase
dc.subjectIsoenzymes
dc.subjectPeptide Chain Initiation, Translational
dc.subjectPolymerase Chain Reaction
dc.subjectPromoter Regions (Genetics)
dc.subjectSaccharomyces cerevisiae
dc.subjectTATA Box
dc.subjectTranscription, Genetic
dc.subjectSaccharomyces cerevisiae
dc.titleIdentification of a functional TATA element in the STA2 glucoamylase gene promoter from Saccharomyces cerevisiae
dc.typeArticle
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