Comparative transcriptomic and proteomic profiling of industrial wine yeast strains

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dc.contributor.authorRossouw, D.en_ZA
dc.contributor.authorVan den Dool, A. H.en_ZA
dc.contributor.authorJacobson, D.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2011-05-15T15:56:31Z
dc.date.available2011-05-15T15:56:31Z
dc.date.issued2010
dc.description.abstractThe geno- and phenotypic diversity of commercial Saccharomyces cerevisiae wine yeast strains provides an opportunity to apply the system-wide approaches that are reasonably well established for laboratory strains to generate insight into the functioning of complex cellular networks in industrial environments. We have previously analyzed the transcriptomes of five industrial wine yeast strains at three time points during alcoholic fermentation. Here, we extend the comparative approach to include an isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomic analysis of two of the previously analyzed wine yeast strains at the same three time points during fermentation in synthetic wine must. The data show that differences in the transcriptomes of the two strains at a given time point rather accurately reflect differences in the corresponding proteomes independently of the gene ontology (GO) category, providing strong support for the biological relevance of comparative transcriptomic data sets in yeast. In line with previous observations, the alignment proves to be less accurate when assessing intrastrain changes at different time points. In this case, differences between the transcriptome and proteome appear to be strongly dependent on the GO category of the corresponding genes. The data in particular suggest that metabolic enzymes and the corresponding genes appear to be strongly correlated over time and between strains, suggesting a strong transcriptional control of such enzymes. The data also allow the generation of hypotheses regarding the molecular origin of significant differences in phenotypic traits between the two strains. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation76
dc.identifier.citation12
dc.identifier.issn992240
dc.identifier.other10.1128/AEM.00586-10
dc.identifier.urihttp://hdl.handle.net/10019.1/9892
dc.subjectAlcoholic fermentation
dc.subjectCellular network
dc.subjectComparative approach
dc.subjectData sets
dc.subjectGene ontology
dc.subjectIn-line
dc.subjectIndustrial environments
dc.subjectMetabolic enzymes
dc.subjectMolecular origins
dc.subjectOn The Go
dc.subjectPhenotypic diversity
dc.subjectPhenotypic traits
dc.subjectProteomes
dc.subjectProteomic analysis
dc.subjectProteomic profiling
dc.subjectSaccharomyces cerevisiae
dc.subjectTime points
dc.subjectTranscriptional control
dc.subjectTranscriptomes
dc.subjectWine yeast
dc.subjectCatalysts
dc.subjectCellular neural networks
dc.subjectEnzymes
dc.subjectFermentation
dc.subjectGenes
dc.subjectIndustry
dc.subjectOntology
dc.subjectWine
dc.subjectYeast
dc.subjectfungal protein
dc.subjectproteome
dc.subjectalcohol
dc.subjectbrewing industry
dc.subjectcommercial species
dc.subjectcomparative study
dc.subjectdata set
dc.subjectenzyme activity
dc.subjectfermentation
dc.subjectgene expression
dc.subjectgenotype
dc.subjecthypothesis testing
dc.subjectmetabolism
dc.subjectmolecular analysis
dc.subjectphenotype
dc.subjectproteomics
dc.subjectspecies diversity
dc.subjectyeast
dc.subjectarticle
dc.subjectbiosynthesis
dc.subjectchemistry
dc.subjectcomparative study
dc.subjectDNA microarray
dc.subjectfermentation
dc.subjectgene expression profiling
dc.subjectgenetics
dc.subjecthigh performance liquid chromatography
dc.subjectmass spectrometry
dc.subjectmicrobiology
dc.subjectwine
dc.subjectyeast
dc.subjectChromatography, High Pressure Liquid
dc.subjectFermentation
dc.subjectFungal Proteins
dc.subjectGene Expression Profiling
dc.subjectIndustrial Microbiology
dc.subjectMass Spectrometry
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectProteome
dc.subjectWine
dc.subjectYeasts
dc.subjectLonchocarpus glaucifolius
dc.subjectSaccharomyces cerevisiae
dc.titleComparative transcriptomic and proteomic profiling of industrial wine yeast strains
dc.typeArticle
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