Biopsy and tracheobronchial aspirates as additional tools for the diagnosis of bovine tuberculosis in living European bison (bison bonasus)

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dc.contributor.authorDidkowska, Annaen_ZA
dc.contributor.authorOrłowska, Blankaen_ZA
dc.contributor.authorWitkowski, Lucjanen_ZA
dc.contributor.authorKatarzyna, Olbrychen_ZA
dc.contributor.authorBrzezinska, Sylwiaen_ZA
dc.contributor.authorAugustynowicz-Kopec, Ewaen_ZA
dc.contributor.authorKrajewska-Wędzina, Monikaen_ZA
dc.contributor.authorBereznowski, Andrzejen_ZA
dc.contributor.authorBielecki, Wojciechen_ZA
dc.contributor.authorKrzysiak, Michałen_ZA
dc.contributor.authorRakowska, Alicjaen_ZA
dc.contributor.authorOlech, Wandaen_ZA
dc.contributor.authorMiller, Michele A.en_ZA
dc.contributor.authorWaters, Wade R.en_ZA
dc.contributor.authorLyashchenko, Konstantin P.en_ZA
dc.contributor.authorAnusz, Krzysztofen_ZA
dc.date.accessioned2022-04-29T06:45:10Z
dc.date.available2022-04-29T06:45:10Z
dc.date.issued2020-11-02
dc.descriptionCITATION: Didkowska, Anna et al. 2020. Biopsy and tracheobronchial aspirates as additional tools for the diagnosis of bovine tuberculosis in living European bison (bison bonasus). Animals, 10(11):2017, doi:10.3390/ani10112017.en_ZA
dc.descriptionThe original publication is available at: https://www.mdpi.com
dc.description.abstractENGLISH ABSTRACT: The diagnosis of bovine tuberculosis (BTB) in living wildlife remains a complex problem, and one of particular importance in endangered species like European bison (Bison bonasus). To identify infection and avoid the unnecessary culling of such valuable individuals, current best practice requires the collection and culture of material from living animals, as mycobacteria isolation remains the gold standard in BTB diagnosis. However, such isolation is challenging due to the need for the immobilization and collection of appropriate clinical material, and because of the sporadic shedding of mycobacteria. In the present study, we evaluated the potential of sampling for the detection of BTB in a group of seven living European bison suspected of being infected with Mycobacterium caprae. The specimens were collected both as swabs from the nasal and pharyngeal cavities, tracheobronchial aspirates (TBA), ultrasound-guided biopsies from lateral retropharyngeal lymph nodes, and post mortem, from mandibular, retropharyngeal and mediastinal lymph nodes. Clinical samples were tested for mycobacterial species via mycobacteriological culture and PCR. M. caprae was isolated from collected material in two out of four living infected individuals (TBA, biopsy) and mycobacterialDNAwas detected in three out of four (TBA, pharyngeal swab) bison. This is the first report of isolation of M. caprae in living European bison. Our findings demonstrate the value of diagnostic tests based on both molecular testing and culture in European bison and confirm the respiratory shedding of viable M. caprae in this host species.en_ZA
dc.description.versionPublisher's version
dc.format.extent10 pagesen_ZA
dc.identifier.citationDidkowska, Anna et al. 2020. Biopsy and tracheobronchial aspirates as additional tools for the diagnosis of bovine tuberculosis in living European bison (bison bonasus). Animals, 10(11):2017, doi:10.3390/ani10112017
dc.identifier.issn2076-2615 (online)
dc.identifier.otherdoi:10.3390/ani10112017
dc.identifier.urihttp://hdl.handle.net/10019.1/124482
dc.language.isoen_ZAen_ZA
dc.publisherMDPIen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectBiopsyen_ZA
dc.subjectEuropean bisonen_ZA
dc.subjectMycobacterium capraeen_ZA
dc.subjectTracheobronchial aspiratesen_ZA
dc.subjectTuberculosisen_ZA
dc.subjectBison bonasusen_ZA
dc.titleBiopsy and tracheobronchial aspirates as additional tools for the diagnosis of bovine tuberculosis in living European bison (bison bonasus)en_ZA
dc.typeArticleen_ZA
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