From steady-state to synchronized yeast glycolytic oscillations I: Model construction

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dc.contributor.authorDu Preez F.B.
dc.contributor.authorVan Niekerk D.D.
dc.contributor.authorKooi B.
dc.contributor.authorRohwer J.M.
dc.contributor.authorSnoep J.L.
dc.date.accessioned2012-08-17T12:32:49Z
dc.date.available2012-08-17T12:32:49Z
dc.date.issued2012
dc.descriptionArticle
dc.description.abstractAn existing detailed kinetic model for the steady-state behavior of yeast glycolysis was tested for its ability to simulate dynamic behavior. Using a small subset of experimental data, the original model was adapted by adjusting its parameter values in three optimization steps. Only small adaptations to the original model were required for realistic simulation of experimental data for limit-cycle oscillations. The greatest changes were required for parameter values for the phosphofructokinase reaction. The importance of ATP for the oscillatory mechanism and NAD(H) for inter-and intra-cellular communications and synchronization was evident in the optimization steps and simulation experiments. In an accompanying paper [du Preez F et al. (2012) FEBS J279, 2823-2836], we validate the model for a wide variety of experiments on oscillatory yeast cells. The results are important for re-use of detailed kinetic models in modular modeling approaches and for approaches such as that used in the Silicon Cell initiative. © 2012 FEBS.
dc.identifier.citationFEBS Journal
dc.identifier.citation279
dc.identifier.citation16
dc.identifier.citation2810
dc.identifier.citation2822
dc.identifier.issn1742464X
dc.identifier.other10.1111/j.1742-4658.2012.08665.x
dc.identifier.urihttp://hdl.handle.net/10019.1/49270
dc.subjectglycolysis
dc.subjectlimit-cycle oscillation
dc.subjectmathematical model
dc.subjectmodel construction
dc.subjectSaccharomyces cerevisiae
dc.titleFrom steady-state to synchronized yeast glycolytic oscillations I: Model construction
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