Karyotypic evolution of Hapalomys inferred from chromosome painting: A detailed characterization contributing new insights into the ancestral Murinae karyotype

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dc.contributor.authorBadenhorst D.
dc.contributor.authorDobigny G.
dc.contributor.authorRobinson T.J.
dc.date.accessioned2012-04-12T08:33:27Z
dc.date.available2012-04-12T08:33:27Z
dc.date.issued2012
dc.description.abstractWe report on the construction of a comparative chromosome map between the emblematic laboratory rat, Rattus norvegicus (RNO), and Delacour's Marmoset rat, Hapalomys delacouri (HDE), based on cross-species fluorescence in situ hybridization with R. norvegicus painting probes. Sixteen R. norvegicus chromosomes (RNO 3-6, 8, 10-15, 17-20, and X) were retained in their entirety (as a conserved block or as a single chromosome) in the H. delacouri genome. The remaining 5 R. norvegicus chromosomes (RNO 1, 2, 7, 9, and 16) produced 2 signals in the H. delacouri karyotype. Our analysis allowed the detection of an X-autosome translocation between RNO X and 11 that occurred convergently in an unrelated species, Bandicota savilei, and a single B chromosome that accounts for the 2n = 48 karyotype observed in this specimen. In total, the rat chromosome paints revealed 27 segments of conserved synteny in H. delacouri. The analysis showed 7 NOR bearing pairs in H. delacouri (HDE 1, 3, 6, 7, 8, 10, and 13) and the occurrence of an interstitial telomeric signal at the centromeric regions of 8 H. delacouri chromosomes (HDE 3, 10, 11, 12, 13, 16, 19, and 22). These data, together with published comparative maps, enabled a revision of the previously postulated murine ancestral condition suggesting that it probably comprised a wholly acrocentric karyotype with 2n = 46-50. Copyright © 2012 S. Karger AG.
dc.identifier.citationCytogenetic and Genome Research
dc.identifier.citation136
dc.identifier.citation2
dc.identifier.citation83
dc.identifier.citation88
dc.identifier.issn14248581
dc.identifier.other10.1159/000335286
dc.identifier.urihttp://hdl.handle.net/10019.1/20701
dc.subjectAncestral karyotype
dc.subjectHapalomys delacouri
dc.subjectMuridae
dc.subjectRattus norvegicus
dc.subjectZoo-FISH
dc.titleKaryotypic evolution of Hapalomys inferred from chromosome painting: A detailed characterization contributing new insights into the ancestral Murinae karyotype
dc.typeArticle
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