Depot-specific and hypercaloric diet-induced effects on the osteoblast and adipocyte differentiation potential of adipose-derived stromal cells

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dc.contributor.authorSadie-Van Gijsen H.
dc.contributor.authorSmith W.
dc.contributor.authordu Toit E.F.
dc.contributor.authorMichie J.
dc.contributor.authorHough F.S.
dc.contributor.authorFerris W.F.
dc.date.accessioned2012-05-17T09:01:01Z
dc.date.available2012-05-17T09:01:01Z
dc.date.issued2012
dc.description.abstractAdipose-derived stromal cells (ADSCs) can be differentiated in vitro into several mesenchyme-derived cell types. We had previously described depot-specific differences in the adipocyte differentiation of ADSCs, and consequently we hypothesized that there may also be depot-specific differences in osteoblast differentiation of ADSCs. For this study, the osteoblast differentiation potential of rat subcutaneous ADSCs (scADSCs) and perirenal visceral ADSCs (pvADSCs) was compared. Osteoblast differentiation media (OM) induced markers of the osteoblastic phenotype in scADSCs, but not in pvADSCs. ADSCs harvested from rats with diet-induced visceral obesity (DIO) exhibited reduced osteoinduction, compared to lean controls, but adipocyte differentiation was not affected. Expression of the pro-osteogenic transcription factor Msx2 was significantly higher in naïve scADSCs from lean and DIO rats than in pvADSCs. Our findings indicate that ADSCs from different anatomical sites are uniquely pre-programmed in vivo in a depot-specific manner, and that diet-induced metabolic disturbances translate into reduced osteoblast differentiation of ADSCs. © 2011 Elsevier Ireland Ltd.
dc.identifier.citationMolecular and Cellular Endocrinology
dc.identifier.citation348
dc.identifier.citation1
dc.identifier.citation55
dc.identifier.citation66
dc.identifier.issn3037207
dc.identifier.other10.1016/j.mce.2011.07.030
dc.identifier.urihttp://hdl.handle.net/10019.1/21091
dc.subjecttranscription factor MSX2
dc.subjectadipocyte
dc.subjectadipose derived stromal cell
dc.subjectanimal cell
dc.subjectarticle
dc.subjectcell differentiation
dc.subjectcontrolled study
dc.subjectin vivo study
dc.subjectintraabdominal fat
dc.subjectmetabolic disorder
dc.subjectnonhuman
dc.subjectobesity
dc.subjectosteoblast
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectstroma cell
dc.subjectsubcutaneous fat
dc.subjectAdipocytes
dc.subjectAdipose Tissue
dc.subjectAlkaline Phosphatase
dc.subjectAnimals
dc.subjectAntigens, Differentiation
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectCell Shape
dc.subjectCells, Cultured
dc.subjectCore Binding Factor Alpha 1 Subunit
dc.subjectEnergy Intake
dc.subjectGene Expression
dc.subjectHomeodomain Proteins
dc.subjectIntegrin-Binding Sialoprotein
dc.subjectMale
dc.subjectObesity
dc.subjectOsteoblasts
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStromal Cells
dc.titleDepot-specific and hypercaloric diet-induced effects on the osteoblast and adipocyte differentiation potential of adipose-derived stromal cells
dc.typeArticle
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