Determination of anthocyanins in wine by direct injection liquid chromatography-diode array detection-mass spectrometry and classification of wines using discriminant analysis

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dc.contributor.authorDe Villiers A.
dc.contributor.authorVanhoenacker G.
dc.contributor.authorMajek P.
dc.contributor.authorSandra P.
dc.date.accessioned2011-05-15T15:59:35Z
dc.date.available2011-05-15T15:59:35Z
dc.date.issued2004
dc.description.abstractA rapid HPLC-diode array detection (DAD) method was developed for the routine analysis of 16 anthocyanins in wine. Direct injection of filtered wine samples followed by selective detection at 520 nm allowed quantitation of these compounds in red wines. The method was linear for malvidin-3-glucoside over the range 5-250 ppm, and the limit of detection for this compound was 0.18 ppm. A volatile mobile phase is used, which enables hyphenation to mass spectrometry (MS). With HPLC-MS, a total of 44 pigments could be identified in South African wines. Obtained mass spectra are discussed for a series of representative wine constituents and results are compared with literature references. An attempt was made to differentiate between different cultivars according to the anthocyanin content using stepwise forward linear discriminant analysis (LDA). © 2004 Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationJournal of Chromatography A
dc.identifier.citation1054
dc.identifier.citation02-Jan
dc.identifier.issn219673
dc.identifier.other10.1016/j.chroma.2004.07.087
dc.identifier.urihttp://hdl.handle.net/10019.1/11261
dc.subjectChromatographic analysis
dc.subjectDiodes
dc.subjectFlow of fluids
dc.subjectFood preservation
dc.subjectLiquid chromatography
dc.subjectMass spectrometry
dc.subjectAnthocyanins
dc.subjectDiode array detection (DAD)
dc.subjectHyphenation
dc.subjectLinear discriminant analysis (LDA)
dc.subjectWine
dc.subjectanthocyanin
dc.subjectglucoside
dc.subjectmalvidin 3 glucoside
dc.subjectunclassified drug
dc.subjectaccuracy
dc.subjectarticle
dc.subjectdiscriminant analysis
dc.subjectfood analysis
dc.subjectfood composition
dc.subjecthigh performance liquid chromatography
dc.subjectliquid chromatography diode array detection
dc.subjectmass spectrometry
dc.subjectpriority journal
dc.subjectred wine
dc.subjectAnthocyanins
dc.subjectChromatography, High Pressure Liquid
dc.subjectDiscriminant Analysis
dc.subjectMass Spectrometry
dc.subjectSpectrophotometry, Ultraviolet
dc.subjectWine
dc.titleDetermination of anthocyanins in wine by direct injection liquid chromatography-diode array detection-mass spectrometry and classification of wines using discriminant analysis
dc.typeArticle
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