Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene in Saccharomyces cerevisiae

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dc.contributor.authorVan Rensburg P.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorPretorius I.S.
dc.date.accessioned2011-05-15T16:01:29Z
dc.date.available2011-05-15T16:01:29Z
dc.date.issued1996
dc.description.abstractA cDNA fragment encoding the Phanerochaete chrysosporium cellobiohydrolase (cbh1-4) was amplified and cloned with the aid of the polymerase chain reaction (PCR) technique. The cbh1-4 gene and the Butyrivibrio fibrisolvens endo-β-1,4-glucanase (end1) gene were successfully expressed in Saccharomyces cerevisiae under the control of the phosphoglycerate kinase-I (PGK1) and alcohol dehydrogenase-II (ADH2) gene promoters and terminators, respectively. The native P. chrysosporium signal sequence mediated secretion of cellobiohydrolase in S. cerevisiae, whereas secretion of the endo-β-1,4-glucanase was directed by the signal sequence of the yeast mating pheromone α-factor (MFα1(S)). These constructs, designated CBH1 and END1, respectively, were expressed separately and jointly in S. cerevisiae. The construction of fur1 ura3 S. cerevisiae strains allowed for the autoselection of these multicopy URA3-based plasmids in rich medium. Enzyme assays confirmed that co-expression of CBH1 and END1 synergistically enhanced cellulose degradation by S. cerevisiae.
dc.description.versionArticle
dc.identifier.citationCurrent Genetics
dc.identifier.citation30
dc.identifier.citation3
dc.identifier.issn1728083
dc.identifier.other10.1007/s002940050128
dc.identifier.urihttp://hdl.handle.net/10019.1/12005
dc.subjectalcohol dehydrogenase
dc.subjectcellulase
dc.subjectcellulose 1,4 beta cellobiosidase
dc.subjectpheromone
dc.subjectphosphoglycerate kinase
dc.subjectsignal peptide
dc.subjectarticle
dc.subjectenzyme assay
dc.subjectmating
dc.subjectnonhuman
dc.subjectphanerochaete
dc.subjectplasmid
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectsaccharomyces cerevisiae
dc.subjectAgaricales
dc.subjectBase Sequence
dc.subjectCellulase
dc.subjectCellulose 1,4-beta-Cellobiosidase
dc.subjectCloning, Molecular
dc.subjectDNA Primers
dc.subjectGene Expression
dc.subjectGenes, Bacterial
dc.subjectGenes, Fungal
dc.subjectGenotype
dc.subjectGlycoside Hydrolases
dc.subjectGram-Negative Anaerobic Bacteria
dc.subjectMolecular Sequence Data
dc.subjectPlasmids
dc.subjectPolymerase Chain Reaction
dc.subjectRecombinant Proteins
dc.subjectRestriction Mapping
dc.subjectSaccharomyces cerevisiae
dc.subjectButyrivibrio fibrisolvens
dc.subjectChrysosporium
dc.subjectPhanerochaete chrysosporium
dc.subjectSaccharomyces cerevisiae
dc.titleCo-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene in Saccharomyces cerevisiae
dc.typeArticle
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