Differential uptake of fumarate by Candida utilis and Schizosaccharomyces pombe

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dc.contributor.authorSaayman M.
dc.contributor.authorVan Vuuren H.J.J.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorViljoen-Bloom M.
dc.date.accessioned2011-05-15T15:56:37Z
dc.date.available2011-05-15T15:56:37Z
dc.date.issued2000
dc.description.abstractThe dicarboxylic acid fumarate is an important intermediate in cellular processes and also serves as a precursor for the commercial production of fine chemicals such as L-malate. Yeast species differ remarkably in their ability to degrade extracellular dicarboxylic acids and to utilise them as their only source of cartoon. In this study we have shown that the yeast Candida utilis effectively degraded extracellular fumarate and L-malate, but glucose or other assimilable carbon sources repressed the transport and degradation of these dicarboxylic acids. The transport of both dicarboxylic acids was shown to be strongly inducible by either fumarate or L-malate while kinetic studies suggest that the two dicarboxylic acids are transported by the same transporter protein. In contrast, Schizosaccharomyces pombe effectively degraded extracellular L-malate, but not fumarate, in the presence of glucose or other assimilable carbon sources. The Sch. pombe malate transporter was unable to transport fumarate, although fumarate inhibited the uptake of L-malate.
dc.description.versionArticle
dc.identifier.citationApplied Microbiology and Biotechnology
dc.identifier.citation54
dc.identifier.citation6
dc.identifier.issn1757598
dc.identifier.other10.1007/s002530000469
dc.identifier.urihttp://hdl.handle.net/10019.1/9950
dc.subjectcarbon
dc.subjectdicarboxylic acid
dc.subjectfumaric acid
dc.subjectglucose
dc.subjectmalic acid
dc.subjectarticle
dc.subjectbiodegradation
dc.subjectCandida utilis
dc.subjection transport
dc.subjectkinetics
dc.subjectnonhuman
dc.subjectSchizosaccharomyces pombe
dc.subjectspecies difference
dc.subjectBiological Transport, Active
dc.subjectCandida
dc.subjectCulture Media
dc.subjectFumarates
dc.subjectGene Expression Regulation, Fungal
dc.subjectMalates
dc.subjectRecombination, Genetic
dc.subjectSaccharomyces cerevisiae
dc.subjectSchizosaccharomyces
dc.titleDifferential uptake of fumarate by Candida utilis and Schizosaccharomyces pombe
dc.typeArticle
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