Effect of gastro-intestinal conditions on the growth of Enterococcus mundtii ST4SA, and production of bacteriocin ST4SA recorded by real-time PCR

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dc.contributor.authorGranger M.
dc.contributor.authorvan Reenen C.A.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T15:56:47Z
dc.date.available2011-05-15T15:56:47Z
dc.date.issued2008
dc.description.abstractEnterococcus mundtii ST4SA, isolated from soybeans, produces a 3950 Da bacteriocin (bacST4SA) active against a variety of Gram-positive and Gram-negative bacteria, including human pathogens. In this study, the effect of gastro-intestinal conditions on the survival of strain ST4SA and production of bacST4SA was studied. Strain ST4SA was cultured in MRS broth at different pH and in MRS broth supplemented with bile, pancreatic enzymes, and contents of the stomach and small intestine of pigs, respectively. After 12 and 24 h at 37 °C, cells were harvested, RNA isolated and cDNA prepared. Expression of the genes encoding bacST4SA, RecA, GroES and 23 S rRNA was studied by real-time PCR (RT-PCR). No significant up- or down-regulation of the genes were recorded, except when cells were grown in MRS at pH 3.5. In this case only RecA and GroES were up-regulated. Growth of strain ST4SA and production of bacST4SA are not affected by conditions in the lower intestine and the strain could be used as a probiotic. © 2008 Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationInternational Journal of Food Microbiology
dc.identifier.citation123
dc.identifier.citation3
dc.identifier.issn1681605
dc.identifier.other10.1016/j.ijfoodmicro.2007.12.009
dc.identifier.urihttp://hdl.handle.net/10019.1/10040
dc.subjectbacteriocin
dc.subjectarticle
dc.subjectbacterial growth
dc.subjectbacterial strain
dc.subjectbacterium isolate
dc.subjectcell growth
dc.subjectEnterococcus
dc.subjectenterococcus mundtii
dc.subjectgastrointestinal tract
dc.subjectgene
dc.subjectgene expression
dc.subjectgroes gene
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpH
dc.subjectreal time polymerase chain reaction
dc.subjectRecA gene
dc.subjectsoybean
dc.subjectBacteriocins
dc.subjectCulture Media
dc.subjectEnterococcus
dc.subjectFood Microbiology
dc.subjectHydrogen-Ion Concentration
dc.subjectPolymerase Chain Reaction
dc.subjectSoybeans
dc.subjectTemperature
dc.subjectTime Factors
dc.subjectEnterococcus mundtii
dc.subjectGlycine max
dc.subjectNegibacteria
dc.subjectPosibacteria
dc.subjectSuidae
dc.titleEffect of gastro-intestinal conditions on the growth of Enterococcus mundtii ST4SA, and production of bacteriocin ST4SA recorded by real-time PCR
dc.typeArticle
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