Nutrient exchange of carbon and nitrogen promotes the formation of stable mutualisms between chlorella sorokiniana and saccharomyces cerevisiae under engineered synthetic growth conditions

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dc.contributor.authorNaidoo, Rene K.en_ZA
dc.contributor.authorSimpson, Zoe F.en_ZA
dc.contributor.authorOosthuizen, Jennifer R.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2019-04-15T08:45:48Z
dc.date.available2019-04-15T08:45:48Z
dc.date.issued2019-04
dc.descriptionCITATION: Naidoo, R. K., et al. 2019. Nutrient exchange of carbon and nitrogen promotes the formation of stable mutualisms between chlorella sorokiniana and saccharomyces cerevisiae under engineered synthetic growth conditions. Frontiers in Microbiology, 10:609, doi:10.3389/fmicb.2019.00609.en_ZA
dc.descriptionThe original publication is available at https://www.frontiersin.orgen_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.description.abstractMicrobial biotechnological processes can be based on single species pure cultures or on multi-species assemblages. While these assemblages can be advantageous by offering more functionalities and more resilience to changing environmental conditions, they can be unpredictable and difficult to control under synthetically engineered growth conditions. To overcome the unpredictable nature of these microbial assemblages, the generation of stable mutualistic systems through synthetic ecology approaches may provide novel solutions for understanding microbial interactions in these environments. Here we establish a stable association between two evolutionarily unrelated, but biotechnologically complementary species isolated from winery wastewater; a strain of the yeast Saccharomyces cerevisiae and microalga, Chlorella sorokiniana. Yeast and microalgae were able to form obligate (interdependent) and non-obligate (facultative) mutualisms under engineered batch co-culture growth conditions. Obligate mutualism was maintained through the reciprocal exchange of carbon and nitrogen where the yeast ferments mannose to produce carbon dioxide for use by the microalga; and the microalga provides the yeast with nitrogen by metabolizing nitrite to ammonium. The effect of temperature and pH on the establishment of these mutualisms was evaluated and pH was found to be a key determinant for mutualism formation under obligatory conditions. Moreover, the combinations of the two species under non-obligatory growth conditions led to improvement in growth rate and biomass production when compared to single species cultures grown under the same conditions. Such engineered mutualisms are the first step in developing stable multi-species assemblages, while providing a system to generate novel insight into the evolution of mutualistic interactions between phylogenetically distant microorganisms.en_ZA
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2019.00609/full
dc.description.versionPublisher's versionen_ZA
dc.format.extent16 pages : illustrationsen_ZA
dc.identifier.citationNaidoo, R. K., et al. 2019. Nutrient exchange of carbon and nitrogen promotes the formation of stable mutualisms between chlorella sorokiniana and saccharomyces cerevisiae under engineered synthetic growth conditions. Frontiers in Microbiology, 10:609, doi:10.3389/fmicb.2019.00609en_ZA
dc.identifier.issn1664-302X (online)
dc.identifier.otherdoi:10.3389/fmicb.2019.00609
dc.identifier.urihttp://hdl.handle.net/10019.1/105562
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers Mediaen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMicrobial biotechnological -- Processingen_ZA
dc.subjectYeast fungi -- Effect of stress onen_ZA
dc.subjectYeast fungi -- Physiologyen_ZA
dc.subjectSaccharomyces cerevisiae -- Effect of stress onen_ZA
dc.subjectSaccharomyces cerevisiae -- Physiologyen_ZA
dc.subjectAlgae -- Cultureen_ZA
dc.subjectChlorella sorokinianaen_ZA
dc.titleNutrient exchange of carbon and nitrogen promotes the formation of stable mutualisms between chlorella sorokiniana and saccharomyces cerevisiae under engineered synthetic growth conditionsen_ZA
dc.typeArticleen_ZA
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