Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis

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dc.contributor.authorStewart E.L.
dc.contributor.authorLiu Z.
dc.contributor.authorCrous P.W.
dc.contributor.authorSzabo L.J.
dc.date.accessioned2011-05-15T15:57:25Z
dc.date.available2011-05-15T15:57:25Z
dc.date.issued1999
dc.description.abstractPartial rDNA sequences were obtained from 26 isolates representing species of Cercospora, Passalora, Paracercospora, Pseudocercospora, Ramulispora, Pseudocercosporella and Mycocentrospora. The combined internal transcribed spacers (ITS) including the 5.8S rRNA gene and 5' end of the 25S gene (primer pairs F63/R635) on rDNA were amplified using PCR and sequenced directly. The ITS regions including the 5.8S varied in length from 502 to 595 bp. The F63/R635 region varied from 508 to 519 bp among isolates sequenced. Reconstructed phylogenies inferred from both regions had highly similar topologies for the taxa examined. Phylogenetic analysis of the sequences resulted in four well-supported clades corresponding to Cercospora, Paracercospora/Pseudocercospora, Passalora and Ramulispora, with bootstrap values greater than 92% for each clade. Based on the results of the analysis, a new combination for Pseudocercosporella aestiva is proposed in Ramulispora, and Paracercospora is reduced to synonymy with Pseudocercospora.
dc.description.versionArticle
dc.identifier.citationMycological Research
dc.identifier.citation103
dc.identifier.citation11
dc.identifier.issn9537562
dc.identifier.other10.1017/S0953756299008680
dc.identifier.urihttp://hdl.handle.net/10019.1/10396
dc.subjectanamorph
dc.subjectinternal transcribed spacer
dc.subjectphylogeny
dc.subjectpolymerase chain reaction
dc.subjectPseudocercosporella aestiva
dc.subjectribosomal DNA
dc.subjectribosomal RNA
dc.subjectsynonymy
dc.subjectfungus
dc.subjectgenetic analysis
dc.subjectphylogenetics
dc.subjectCercospora
dc.subjectMycocentrospora
dc.subjectMycosphaerella
dc.subjectParacercospora
dc.subjectPassalora
dc.subjectPseudocercospora
dc.subjectPseudocercosporella
dc.subjectRamulispora
dc.titlePhylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis
dc.typeArticle
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