Genotyping of Mycobacterium tuberculosis with additional markers enhances accuracy in epidemiological studies

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dc.contributor.authorWarren R.
dc.contributor.authorRichardson M.
dc.contributor.authorSampson S.
dc.contributor.authorHauman J.H.
dc.contributor.authorBeyers N.
dc.contributor.authorDonald P.R.
dc.contributor.authorVan Helden P.D.
dc.date.accessioned2011-05-15T15:56:58Z
dc.date.available2011-05-15T15:56:58Z
dc.date.issued1996
dc.description.abstractTwo highly polymorphic Mycobacterium tuberculosis genomic domains, characterized by hybridization to the oligonucleotide (GTG)5, were identified as potential DNA fingerprinting probes. These domains were cloned [pMTB484(1) and pMTB484(2K4), respectively] and shown to be useful for genotype analysis by Southern blotting. These probes were used to genotype geographically linked strains of M. tuberculosis previously shown to have identical IS6110 fingerprints. Subsequent DNA fingerprints generated with MTB484(1) and MTB484(2K4) showed a high degree of polymorphism, allowing subclassification of IS6110-defined clusters into composites of smaller clusters and unique strains. Correlation of the molecular data with patient interviews and clinical records confirmed the sensitivity of these probes, as contacts were established only within subclusters. These findings demonstrate the requirement for multiple probes to accurately classify M. tuberculosis strains, even those with high copy numbers of IS6110. The enhanced accuracy of strain typing should, in turn, further our understanding of the epidemiology of tuberculosis.
dc.description.versionArticle
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation34
dc.identifier.citation9
dc.identifier.issn951137
dc.identifier.urihttp://hdl.handle.net/10019.1/10143
dc.subjectantibody specificity
dc.subjectarticle
dc.subjectdiagnostic accuracy
dc.subjectdna fingerprinting
dc.subjectgene cluster
dc.subjectgenetic recombination
dc.subjectgenotype
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmycobacterium tuberculosis
dc.subjectpriority journal
dc.subjectprotein domain
dc.subjectrestriction fragment length polymorphism
dc.subjecttandem repeat
dc.subjecttuberculosis
dc.subjectBacterial Typing Techniques
dc.subjectBiological Markers
dc.subjectDNA Fingerprinting
dc.subjectDNA, Bacterial
dc.subjectGenome, Bacterial
dc.subjectMolecular Probe Techniques
dc.subjectMycobacterium tuberculosis
dc.subjectMycobacterium
dc.subjectMycobacterium tuberculosis
dc.titleGenotyping of Mycobacterium tuberculosis with additional markers enhances accuracy in epidemiological studies
dc.typeArticle
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