Characterization of a 3944 Da bacteriocin, produced by Enterococcus mundtii ST15, with activity against Gram-positive and Gram-negative bacteria

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dc.contributor.authorDe Kwaadsteniet M.
dc.contributor.authorTodorov S.D.
dc.contributor.authorKnoetze H.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T15:56:47Z
dc.date.available2011-05-15T15:56:47Z
dc.date.issued2005
dc.description.abstractStrain ST15, isolated from soy beans, and identified as Enterococcus mundtii, produces a 3944 Da bacteriocin that inhibits the growth of Lactobacillus sakei, Enterococcus faecalis, Bacillus cereus, Propionibacterium sp., Clostridium tyrobutyricum, Acinetobacter baumanii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae and Streptococcus caprinus. Bacteriocin ST15 is inactivated by proteinase K, pronase, pepsin, protease and Triton X-114, but not when treated with catalase, α-amylase, Triton X-100, SDS, Tween 20, Tween 80, urea and EDTA. No change in activity was recorded after 2 h at pH values between 2.0 and 12.0, and after treatment at 100°C for 90 min. Activity was, however, lost after treatment at 121°C for 20 min. The mode of activity is bactericidal. The highest level of activity (51 200 AU ml-1) was recorded when cells were grown in MRS broth, pH 6.5. Bacteriocin ST15 differs from other broad-spectrum bacteriocins described for Enterococcus spp. by being active against Gram-negative bacteria and by being smaller. © 2005 Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationInternational Journal of Food Microbiology
dc.identifier.citation105
dc.identifier.citation3
dc.identifier.issn1681605
dc.identifier.other10.1016/j.ijfoodmicro.2005.03.021
dc.identifier.urihttp://hdl.handle.net/10019.1/10045
dc.subjectamylase
dc.subjectbacterial protein
dc.subjectbacteriocin
dc.subjectcatalase
dc.subjectdodecyl sulfate sodium
dc.subjectedetic acid
dc.subjectpepsin A
dc.subjectpolysorbate 20
dc.subjectpolysorbate 80
dc.subjectpronase
dc.subjectproteinase
dc.subjectproteinase K
dc.subjecttriton x 100
dc.subjecttriton x 114
dc.subjecturea
dc.subjectAcinetobacter baumannii
dc.subjectantibacterial activity
dc.subjectarticle
dc.subjectBacillus cereus
dc.subjectbacterial growth
dc.subjectbacterial strain
dc.subjectbactericidal activity
dc.subjectbacterium isolation
dc.subjectcell growth
dc.subjectClostridium
dc.subjectclostridium tyrobutyricum
dc.subjectEnterococcus
dc.subjectEnterococcus faecalis
dc.subjectenterococcus mundtii
dc.subjectGram negative bacterium
dc.subjectGram positive bacterium
dc.subjectKlebsiella pneumoniae
dc.subjectLactobacillus sakei
dc.subjectnonhuman
dc.subjectpH
dc.subjectPropionibacterium
dc.subjectprotein analysis
dc.subjectprotein function
dc.subjectPseudomonas aeruginosa
dc.subjectsoybean
dc.subjectStaphylococcus aureus
dc.subjectStreptococcus caprinus
dc.subjectStreptococcus pneumoniae
dc.subjecttemperature
dc.subjecttime
dc.subjectAdsorption
dc.subjectAntibiosis
dc.subjectBacteriocins
dc.subjectEnterococcus
dc.subjectFood Microbiology
dc.subjectGram-Negative Bacteria
dc.subjectGram-Positive Bacteria
dc.subjectHydrogen-Ion Concentration
dc.subjectMolecular Weight
dc.subjectTemperature
dc.subjectTime Factors
dc.subjectAcinetobacter baumannii
dc.subjectBacillus cereus
dc.subjectBacteria (microorganisms)
dc.subjectClostridium
dc.subjectClostridium tyrobutyricum
dc.subjectEnterococcus
dc.subjectEnterococcus faecalis
dc.subjectEnterococcus mundtii
dc.subjectGlycine max
dc.subjectKlebsiella pneumoniae
dc.subjectLactobacillus sakei
dc.subjectNegibacteria
dc.subjectPosibacteria
dc.subjectPropionibacterium
dc.subjectPseudomonas aeruginosa
dc.subjectStaphylococcus aureus
dc.subjectStreptococcus gallolyticus
dc.subjectStreptococcus pneumoniae
dc.titleCharacterization of a 3944 Da bacteriocin, produced by Enterococcus mundtii ST15, with activity against Gram-positive and Gram-negative bacteria
dc.typeArticle
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