Barcoding and microcoding using "identiprimers" with Leptographium species

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dc.contributor.authorVan Zuydam N.R.
dc.contributor.authorPaciura D.
dc.contributor.authorJacobs K.
dc.contributor.authorWingfield M.J.
dc.contributor.authorCoetzee M.P.
dc.contributor.authorWingfield B.D.
dc.date.accessioned2011-05-15T16:00:02Z
dc.date.available2011-05-15T16:00:02Z
dc.date.issued2010
dc.description.abstractLeptographium species provide an ideal model to test the applications of a PCR microcoding system for differentiating species of other genera of ascomycetes. Leptographium species are closely related and share similar gross morphology. Probes designed for a PhyloChip for Leptographium have been transferred and tested as primers for PCR diagnostic against Leptographium species. The primers were combined with complementary universal primers to identify known and suspected undescribed species of Leptographium. The primer set was optimized for 56 species, including the three varieties of L. wageneri, then blind-tested against 10 random DNA samples. The protocols established in this study successfully identified species from the blind test as well as eight previously undescribed isolates of Leptographium. The undescribed isolates were identified as new species of Leptographium with the aid of the microcoding PCR identification system established in this study. The primers that were positive for each undescribed isolate were used to determine close relatives of these species and some of their biological characteristics. The transfer of oligonucleotides from a micro-array platform to a PCR diagnostic was successful, and the identification system is robust for both known and unknown species of Leptographium. © 2010 by The Mycological Society of America.
dc.description.versionArticle
dc.identifier.citationMycologia
dc.identifier.citation102
dc.identifier.citation6
dc.identifier.issn275514
dc.identifier.other10.3852/09-291
dc.identifier.urihttp://hdl.handle.net/10019.1/11494
dc.subjectprimer DNA
dc.subjectfungus
dc.subjectgenetic analysis
dc.subjectidentification method
dc.subjectmorphology
dc.subjectpolymerase chain reaction
dc.subjectarticle
dc.subjectAscomycetes
dc.subjectclassification
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmolecular genetics
dc.subjectphylogeny
dc.subjectpolymerase chain reaction
dc.subjectAscomycota
dc.subjectDNA Primers
dc.subjectMolecular Sequence Data
dc.subjectPhylogeny
dc.subjectPolymerase Chain Reaction
dc.subjectAscomycota
dc.subjectLeptographium
dc.subjectLeptographium wageneri
dc.titleBarcoding and microcoding using "identiprimers" with Leptographium species
dc.typeArticle
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