Characterization and quantitation of alpha-adrenergic receptor subtypes in rat hypothalamus

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dc.contributor.authorNeethling A.C.
dc.contributor.authorMcCarthy B.W.
dc.contributor.authorTaljaard J.J.F.
dc.date.accessioned2011-05-15T16:01:19Z
dc.date.available2011-05-15T16:01:19Z
dc.date.issued1981
dc.description.abstractThe binding of [3H]dihydroergocryptine ([3H]-DHE) to rat hypothalamic membranes was examined. Displacement of [3H]-DHE by 100 nM phentolamine, prazosin and clonidine can be used to assay for total α-, α1- and α2-adrenergic receptor sites respectively. The α1-adrenergic receptor content of the hypothalamus is 4.1 pmoles/g tissue whereas the α2-level is 6.5 pmoles/g tissue. [3H]-WB 4101 (α1 selective) and [3H]clonidine (α2 selective) binding yielded similar levels of 3.2 and 5.8 pmoles/g tissue. It is concluded that [3H]-DHE is a suitable ligand for the assay of α-adrenergic receptor subtypes under the conditions determined in this study.
dc.description.versionArticle
dc.identifier.citationBiochemical Pharmacology
dc.identifier.citation30
dc.identifier.citation6
dc.identifier.issn62952
dc.identifier.other10.1016/0006-2952(81)90127-1
dc.identifier.urihttp://hdl.handle.net/10019.1/11915
dc.subjectalpha adrenergic receptor
dc.subjectapomorphine
dc.subjectdopamine
dc.subjecthaloperidol
dc.subjectnoradrenalin
dc.subjectphentolamine
dc.subjectpimozide
dc.subjectprazosin
dc.subjectradioisotope
dc.subjecttritium
dc.subjectyohimbine
dc.subject2 [2 (2,6 dimethoxyphenoxy)ethylaminomethyl] 1,4 benzodioxane h 3
dc.subjectanimal experiment
dc.subjectcentral nervous system
dc.subjectclonidine h 3
dc.subjectdihydroergocryptine h 3
dc.subjectdrug binding
dc.subjectendocrine system
dc.subjecthypothalamus
dc.subjectin vitro study
dc.subjectnervous system
dc.subjectrat
dc.subjectAnimal
dc.subjectBinding Sites
dc.subjectClonidine
dc.subjectDihydroergotoxine
dc.subjectFemale
dc.subjectHypothalamus
dc.subjectNorepinephrine
dc.subjectPhentolamine
dc.subjectPrazosin
dc.subjectRats
dc.subjectReceptors, Adrenergic
dc.subjectReceptors, Adrenergic, alpha
dc.subjectSupport, Non-U.S. Gov't
dc.titleCharacterization and quantitation of alpha-adrenergic receptor subtypes in rat hypothalamus
dc.typeArticle
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