Isolation and selection of Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish

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dc.contributor.authorLalloo R.
dc.contributor.authorRamchuran S.
dc.contributor.authorRamduth D.
dc.contributor.authorGorgens J.
dc.contributor.authorGardiner N.
dc.date.accessioned2011-05-15T15:59:24Z
dc.date.available2011-05-15T15:59:24Z
dc.date.issued2007
dc.description.abstractAims: To isolate, select and evaluate Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. Methods and Results: Natural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibition of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium, nitrite, nitrate and phosphate ions. Based on suitability to predefined characteristics, the isolates B001, B002 and B003 were selected and evaluated in vitro in the presence of Aer. hydrophila and in a preliminary in vivo trial with C. carpio. The inhibitory effect on pathogen growth and the decrease in concentrations of waste ions was demonstrated. Based on 16S RNA sequence homology, the isolates were identified as Bacillus subtilis, Bacillus cereus and Bacillus licheniformis, respectively. Isolate B002 did not contain the anthrax virulence plasmids pOX1, pOX2 or the B. cereus enterotoxin. Conclusions: Selected isolates effected synergistic reduction in pathogen load and the concentrations of waste ions in vitro and in vivo and are safe for use in ornamental aquaculture. Significance and Impact of the Study: A new approach for assessment of biological agents was demonstrated and has yielded putative isolates for development into aquaculture products. © 2007 CSIR.
dc.description.versionArticle
dc.identifier.citationJournal of Applied Microbiology
dc.identifier.citation103
dc.identifier.citation5
dc.identifier.issn13645072
dc.identifier.other10.1111/j.1365-2672.2007.03360.x
dc.identifier.urihttp://hdl.handle.net/10019.1/11155
dc.subjectammonium nitrate
dc.subjectenterotoxin
dc.subjectnitrate
dc.subjectphosphate
dc.subjectbacterium
dc.subjectcyprinid
dc.subjectfish culture
dc.subjectgenetic analysis
dc.subjectinhibition
dc.subjection
dc.subjectornamental species
dc.subjectpathogen
dc.subjectplasmid
dc.subjecttoxin
dc.subjectvirulence
dc.subjectwater quality
dc.subjectAeromonas hydrophila
dc.subjectanthrax
dc.subjectarticle
dc.subjectBacillus cereus
dc.subjectBacillus licheniformis
dc.subjectBacillus subtilis
dc.subjectbacterial growth
dc.subjectbacterial virulence
dc.subjectbacterium contamination
dc.subjectbiotechnology
dc.subjectcarp
dc.subjectcontrolled study
dc.subjectgrowth inhibition
dc.subjectin vitro study
dc.subjectnonhuman
dc.subjectpisciculture
dc.subjectplasmid
dc.subjectRNA sequence
dc.subjectsequence homology
dc.subjectwater quality
dc.subjectAeromonas hydrophila
dc.subjectAmmonia
dc.subjectAnimals
dc.subjectAnimals, Domestic
dc.subjectBacillus
dc.subjectBacterial Infections
dc.subjectCarps
dc.subjectFish Diseases
dc.subjectFishes
dc.subjectIons
dc.subjectNitrates
dc.subjectNitrites
dc.subjectPest Control, Biological
dc.subjectPhosphates
dc.subjectRibotyping
dc.subjectWater Microbiology
dc.subjectWater Purification
dc.subjectAeromonas hydrophila
dc.subjectBacillus (bacterium)
dc.subjectBacillus cereus
dc.subjectBacillus licheniformis
dc.subjectBacillus subtilis
dc.subjectBacteria (microorganisms)
dc.subjectCyprinus carpio
dc.titleIsolation and selection of Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish
dc.typeArticle
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