Differentiation of Leuconostoc species by nicotinamide adenine dinucleotide-dependent D(-)-lactic dehydrogenase profiles

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dc.contributor.authorDicks L.M.T.
dc.contributor.authorVan Vuuren H.J.J.
dc.date.accessioned2011-05-15T16:04:58Z
dc.date.available2011-05-15T16:04:58Z
dc.date.issued1990
dc.description.abstractThe electrophoretic mobility of the D(-)-nLDH's of Leuconostoc oenos (nine strains), Leuconostoc mesenteroides subsp. mesenteroides (one strain), Leuconostoc mesenteroides subsp. dextranicum (one strain), Leuconostoc lactis (three strains), Leuconostoc paramesenteroides (two strains) and a Leuconostoc sp. were compared using conventional and stacked polyacrylamide gels. A single diffused D(-)-nLDH band was obtained for each strain on a 7% acrylamide gel. However, several well defined D(-)-nLDH bands were obtained for each strain when a polyacrylamide stacked gel was used. Based on D(-)-nLDH profiles, strains of L. oenos were divided into two subgroups. The overall D(-)-nLDH profiles of strains of L. oenos were different from those obtained for the non-acidophilic Leuconostoc spp. Leuconostoc mesenteroides subsp. mesenteroides and L. mesenteroides subsp. dextranicum had similar D(-)-nLDH profiles. The overall D(-)-nLDH profiles of L. lactis corresponded well. Different D(-)-nLDH profiles were obtained for L. paramesenteroides DSM 20201 and the type strain of L. paramesenteroides (DSM 20288(T)). The D(-)-nLDH profile of Leuconostoc sp. ATCC 21436 corresponded well with the profile obtained for the D(-)-nLDH electrophoretic marker of Lactobacillus leichmannii.
dc.description.versionArticle
dc.identifier.citationFEMS Microbiology Letters
dc.identifier.citation67
dc.identifier.citation02-Jan
dc.identifier.issn3781097
dc.identifier.urihttp://hdl.handle.net/10019.1/12907
dc.subjectlactate dehydrogenase
dc.subjectnicotinamide adenine dinucleotide
dc.subjectarticle
dc.subjectbacterium identification
dc.subjectleuconostoc
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectBacteria (microorganisms)
dc.subjectLactobacillus leichmannii
dc.subjectLeuconostoc
dc.subjectLeuconostoc lactis
dc.subjectLeuconostoc mesenteroides subsp. dextranicum
dc.subjectLeuconostoc mesenteroides subsp. mesenteroides
dc.subjectOenococcus oeni
dc.subjectWeissella paramesenteroides
dc.titleDifferentiation of Leuconostoc species by nicotinamide adenine dinucleotide-dependent D(-)-lactic dehydrogenase profiles
dc.typeArticle
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