Stable methylation patterns in interspecific antelope hybrids and the characterization and localization of a satellite fraction in the Alcelaphini and Hippotragini

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dc.contributor.authorRobinson T.J.
dc.contributor.authorWittekindt O.
dc.contributor.authorPasantes J.J.
dc.contributor.authorModi W.S.
dc.contributor.authorSchempp W.
dc.contributor.authorMorris-Rosendahl D.J.
dc.date.accessioned2011-05-15T15:59:05Z
dc.date.available2011-05-15T15:59:05Z
dc.date.issued2000
dc.description.abstractConflicting data has recently appeared concerning altered methylation patterns in interspecific mammalian hybrids and the potential this may hold for driving karyotypic evolution. We report no detectable methylation difference in the genomic DNA of different interspecific F1 antelope hybrids (family Bovidae) and their parent species using the methylation-sensitive enzyme HpaII and its methylation insensitive isoschizomer MspI. However, both enzymes released a tandemly repeated satellite array. Characterization of the repeat using Southern blotting and a combination of sequencing, fluorescence in-situ hybridization (FISH) and C-banding, shows some similarity in the family of repeats between the hybridizing antelope species groups, and that the satellite is localized in the centromeric C-band positive regions of the chromosomes. Moreover, although there is little meaningful sequence homology with the well characterized bovine 1.715 satellite DNA, there is 86% sequence similarity with the sheep/goat satellite I, suggesting that they are related and are likely to have originated and evolved separately from the bovine unit.
dc.description.versionArticle
dc.identifier.citationChromosome Research
dc.identifier.citation8
dc.identifier.citation7
dc.identifier.issn9673849
dc.identifier.other10.1023/A:1009294226213
dc.identifier.urihttp://hdl.handle.net/10019.1/10992
dc.subjectsatellite DNA
dc.subjectanimal tissue
dc.subjectantelope
dc.subjectarticle
dc.subjectcentromere
dc.subjectfluorescence in situ hybridization
dc.subjectgene sequence
dc.subjecthybrid
dc.subjectisoschizomer
dc.subjectkaryotype evolution
dc.subjectmethylation
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectsequence homology
dc.subjectSouthern blotting
dc.subjectspecies difference
dc.subjecttandem repeat
dc.subjectAnimals
dc.subjectAntelopes
dc.subjectBlotting, Southern
dc.subjectCells, Cultured
dc.subjectCentromere
dc.subjectChromosome Banding
dc.subjectCloning, Molecular
dc.subjectCrosses, Genetic
dc.subjectDeoxyribonuclease HpaII
dc.subjectDNA Methylation
dc.subjectDNA, Satellite
dc.subjectEvolution, Molecular
dc.subjectFemale
dc.subjectHybridization, Genetic
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMale
dc.subjectMolecular Sequence Data
dc.subjectTandem Repeat Sequences
dc.subjectAlcelaphinae
dc.subjectAnimalia
dc.subjectAntelope
dc.subjectBovidae
dc.subjectBovinae
dc.subjectCapra hircus
dc.subjectMammalia
dc.subjectOvis aries
dc.titleStable methylation patterns in interspecific antelope hybrids and the characterization and localization of a satellite fraction in the Alcelaphini and Hippotragini
dc.typeArticle
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