The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A).

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dc.contributor.advisorSwart, Pieteren_ZA
dc.contributor.advisorSwart, Amanda C.en_ZA
dc.contributor.authorSwart, Liezel Mariaen_ZA
dc.contributor.otherStellenbosch University. Faculty of Science. Dept. of Biochemistry.en_ZA
dc.date.accessioned2017-02-20T14:51:23Z
dc.date.accessioned2017-03-29T12:08:15Z
dc.date.available2017-02-20T14:51:23Z
dc.date.available2017-03-29T12:08:15Z
dc.date.issued2017-03
dc.descriptionThesis (MSc)--Stellenbosch University, 2017.en_ZA
dc.description.abstractENGLISH ABSTRACT: The study describes: . The synthesis of a racemic mixture of Compound A (2-(4-acetoxyphenyl)-2-chloro-N-methylethylammonium chloride), a synthetic analogue of the active compound isolated from the African shrub, Salsola tuberculataformis Botschantzev. . The development of a strategy to separate the R- and S-enantiomers of Compound A. . The isolation and purification of a highly purified substrate-free cytochrome P450 11β-hydroxylase (CYP11B1) from ovine adrenals. . The isolation of a partially purified mixture of the mitochondrial electron transport chain, adrenodoxin reductase and adrenodoxin from ovine adrenals. . The investigation into the mechanism of action of the R- and S-enantiomers of Compound A on the spectroscopic properties of substrate-free CYP11B1. . The investigation into the influence of the R- and S-enantiomers of Compound A on the mitochondrial electron transport chain by using cytochrome c as an electron acceptor.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMNG: Hierdie studie beskryf: . Die sintese van ‘n rasemiese mengsel van Verbinding A (2-(4-asetoksifeniel)-2-chloro-N-metielammoniumchloried), ‘n sintetiese analoog van die aktiewe molecule wat in die Suider-Afrikaanse plant, Salsola tuberculataformis Botschantzev. . Die ontwikkeling van 'n strategie om van die R- en S-enantiomere van Verbinding A te skei. . Die isolering en suiwering van ‘n hoogs gesuiwerde, substraat-vrye sitochroom P450 11β-hidroksilase (CYP11B1) uit skaap-byniere. . Die isolering van ‘n gedeeltelike gesuiwerde mengsel van die mitokondriale elektrontransportketting, adrenodoksienreduktase en adrenodoksien uit skaapbyniere. . ‘n Ondersoek na die meganisme van werking van die R- en S-enantiomere van Verbinding A op die spektroskopiese eienskappe van ‘n gesuiwerde substraat-vrye CYP11B1. . ‘n Ondersoek na die invloed van die R- en S-enantiomere van Verbinding A op die mitokondriale elektrontransportketting met behulp van sitochroom c as 'n elektronakseptor.af_ZA
dc.format.extent134 pages : illustrationsen_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/101101
dc.language.isoen_ZAen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.rights.holderStellenbosch Universityen_ZA
dc.subjectSalsola tuberculata -- Namibiaen_ZA
dc.subjectSalsola tuberculata -- Contraceptive propertiesen_ZA
dc.subjectAdrenal steriodogenesis and cytochrome enzymesen_ZA
dc.subjectMitrochondrial electron transport chain isolationen_ZA
dc.subjectKarakul ewes -- Gestationen_ZA
dc.subjectKarakul sheep -- Abnormalitiesen_ZA
dc.subjectUCTDen_ZA
dc.titleThe biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A).en_ZA
dc.typeThesisen_ZA
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Doctoral Degrees (Biochemistry)