The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A).

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Stellenbosch : Stellenbosch University
ENGLISH ABSTRACT: The study describes: . The synthesis of a racemic mixture of Compound A (2-(4-acetoxyphenyl)-2-chloro-N-methylethylammonium chloride), a synthetic analogue of the active compound isolated from the African shrub, Salsola tuberculataformis Botschantzev. . The development of a strategy to separate the R- and S-enantiomers of Compound A. . The isolation and purification of a highly purified substrate-free cytochrome P450 11β-hydroxylase (CYP11B1) from ovine adrenals. . The isolation of a partially purified mixture of the mitochondrial electron transport chain, adrenodoxin reductase and adrenodoxin from ovine adrenals. . The investigation into the mechanism of action of the R- and S-enantiomers of Compound A on the spectroscopic properties of substrate-free CYP11B1. . The investigation into the influence of the R- and S-enantiomers of Compound A on the mitochondrial electron transport chain by using cytochrome c as an electron acceptor.
AFRIKAANSE OPSOMMNG: Hierdie studie beskryf: . Die sintese van ‘n rasemiese mengsel van Verbinding A (2-(4-asetoksifeniel)-2-chloro-N-metielammoniumchloried), ‘n sintetiese analoog van die aktiewe molecule wat in die Suider-Afrikaanse plant, Salsola tuberculataformis Botschantzev. . Die ontwikkeling van 'n strategie om van die R- en S-enantiomere van Verbinding A te skei. . Die isolering en suiwering van ‘n hoogs gesuiwerde, substraat-vrye sitochroom P450 11β-hidroksilase (CYP11B1) uit skaap-byniere. . Die isolering van ‘n gedeeltelike gesuiwerde mengsel van die mitokondriale elektrontransportketting, adrenodoksienreduktase en adrenodoksien uit skaapbyniere. . ‘n Ondersoek na die meganisme van werking van die R- en S-enantiomere van Verbinding A op die spektroskopiese eienskappe van ‘n gesuiwerde substraat-vrye CYP11B1. . ‘n Ondersoek na die invloed van die R- en S-enantiomere van Verbinding A op die mitokondriale elektrontransportketting met behulp van sitochroom c as 'n elektronakseptor.
Thesis (MSc)--Stellenbosch University, 2017.
Salsola tuberculata -- Namibia, Salsola tuberculata -- Contraceptive properties, Adrenal steriodogenesis and cytochrome enzymes, Mitrochondrial electron transport chain isolation, Karakul ewes -- Gestation, Karakul sheep -- Abnormalities, UCTD