Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography
dc.contributor.author | de Beer D. | |
dc.contributor.author | Jerz G. | |
dc.contributor.author | Joubert E. | |
dc.contributor.author | Wray V. | |
dc.contributor.author | Winterhalter P. | |
dc.date.accessioned | 2011-05-15T15:59:34Z | |
dc.date.available | 2011-05-15T15:59:34Z | |
dc.date.issued | 2009 | |
dc.description.abstract | Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy. © 2009 Elsevier B.V. All rights reserved. | |
dc.description.version | Article | |
dc.identifier.citation | Journal of Chromatography A | |
dc.identifier.citation | 1216 | |
dc.identifier.citation | 19 | |
dc.identifier.issn | 219673 | |
dc.identifier.other | 10.1016/j.chroma.2009.02.056 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/11252 | |
dc.subject | Counter-current chromatography | |
dc.subject | Cyclopia subternata | |
dc.subject | Fabaceae | |
dc.subject | Isomangiferin | |
dc.subject | Mangiferin | |
dc.subject | NMR spectroscopy | |
dc.subject | Xanthones | |
dc.subject | Acetonitrile | |
dc.subject | Body fluids | |
dc.subject | Bubbles (in fluids) | |
dc.subject | Chromatographic analysis | |
dc.subject | Chromatography | |
dc.subject | Electrospray ionization | |
dc.subject | Esters | |
dc.subject | Ethers | |
dc.subject | High performance liquid chromatography | |
dc.subject | High pressure liquid chromatography | |
dc.subject | Liquids | |
dc.subject | Methanol | |
dc.subject | Nuclear magnetic resonance | |
dc.subject | Nuclear magnetic resonance spectroscopy | |
dc.subject | Organic solvents | |
dc.subject | Phenols | |
dc.subject | Solvent extraction | |
dc.subject | acetic acid ethyl ester | |
dc.subject | coumaric acid | |
dc.subject | eriocitrin | |
dc.subject | hesperidin | |
dc.subject | isomangiferin | |
dc.subject | luteolin | |
dc.subject | mangiferin | |
dc.subject | methanol | |
dc.subject | phenol derivative | |
dc.subject | scolymoside | |
dc.subject | unclassified drug | |
dc.subject | water | |
dc.subject | article | |
dc.subject | carbon nuclear magnetic resonance | |
dc.subject | counter current chromatography | |
dc.subject | Cyclopia subternata | |
dc.subject | drug isolation | |
dc.subject | drug structure | |
dc.subject | electrospray mass spectrometry | |
dc.subject | extraction | |
dc.subject | heteronuclear multiple bond correlation | |
dc.subject | high performance liquid chromatography | |
dc.subject | medicinal plant | |
dc.subject | nonhuman | |
dc.subject | plant leaf | |
dc.subject | priority journal | |
dc.subject | proton nuclear magnetic resonance | |
dc.subject | Chromatography, High Pressure Liquid | |
dc.subject | Countercurrent Distribution | |
dc.subject | Cyclopia Plant | |
dc.subject | Methanol | |
dc.subject | Nuclear Magnetic Resonance, Biomolecular | |
dc.subject | Plant Extracts | |
dc.subject | Xanthones | |
dc.subject | Cyclopia subternata | |
dc.subject | Fabaceae | |
dc.title | Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography | |
dc.type | Article |