Isolation and characterization of the TIM10 homologue from the yeast Pichia sorbitophila a putative component of the mitochondrial protein import system

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dc.contributor.authorKayingo G.
dc.contributor.authorPotier S.
dc.contributor.authorHohmann S.
dc.contributor.authorPrior B.A.
dc.date.accessioned2011-05-15T16:05:34Z
dc.date.available2011-05-15T16:05:34Z
dc.date.issued2000
dc.description.abstractThe Saccharomyces cerevisiae TIM10 gene encodes one of the few essential mitochondrial proteins that are required for the import of nuclear-encoded precursor proteins from the cytosol and their subsequent sorting into the different mitochondrial compartments. We have isolated and characterized a putative homologue of TIM10 from the halotolerant yeast Pichia sorbitophila. The Pichia TIM10 gene encodes a protein of 90 amino acids with 66% identity to S. cerevisiae Tim10p. It was capable of suppresing the temperature sensitivity of tim10-1 mutant in S. cerevisiae, suggesting that Pichia TIM10 is both a functional and structural homologue of S. cerevisiae TIM10. The putative Pichia TIM10 gene product contains all the four conserved cysteine residues and the two CX3C motifs typical of the Tim family proteins in the mitochondrial intermembrances space. Using anti Tim10p serum, Western blots detected a protein of about 10 kDa, suggesting that the Pichia Tim10p is a mitochondrial protein. The results suggest that mitochondrial import and sorting systems might be also strongly conserved in other fungi. The coding sequence of the P. sorbitophila TIM10 has been deposited in the EMBL Nucleotide Sequence Database under Accession No. AJ243940. Copyright (C) 2000 John Wiley and Sons, Ltd.
dc.description.versionArticle
dc.identifier.citationYeast
dc.identifier.citation16
dc.identifier.citation7
dc.identifier.issn0749503X
dc.identifier.other10.1002/(SICI)1097-0061(200005)16:7<589::AID-YEA555>3.0.CO;2-G
dc.identifier.urihttp://hdl.handle.net/10019.1/13195
dc.subjectantiserum
dc.subjectcysteine
dc.subjectfungal protein
dc.subjectgene product
dc.subjectamino acid sequence
dc.subjectarticle
dc.subjectcytosol
dc.subjectgene isolation
dc.subjectgenetic analysis
dc.subjectimmunoblotting
dc.subjectmitochondrion
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectPichia
dc.subjectPichia sorbitophila
dc.subjectpriority journal
dc.subjectprotein transport
dc.subjectSaccharomyces cerevisiae
dc.subjectsequence homology
dc.subjecttemperature sensitive mutant
dc.subjecttemperature sensitivity
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectBlotting, Western
dc.subjectCross Reactions
dc.subjectFungal Proteins
dc.subjectGenes, Fungal
dc.subjectGenetic Complementation Test
dc.subjectMembrane Proteins
dc.subjectMitochondria
dc.subjectMolecular Sequence Data
dc.subjectPhylogeny
dc.subjectPichia
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectSequence Analysis, DNA
dc.titleIsolation and characterization of the TIM10 homologue from the yeast Pichia sorbitophila a putative component of the mitochondrial protein import system
dc.typeArticle
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