Isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum

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dc.contributor.authorVan Reenen C.A.
dc.contributor.authorDicks L.M.T.
dc.contributor.authorChikindas M.L.
dc.date.accessioned2011-05-15T15:59:25Z
dc.date.available2011-05-15T15:59:25Z
dc.date.issued1998
dc.description.abstractLactobacillus plantarum 423, isolated from sorghum beer, produces a bacteriocin (plantaricin 423) which is inhibitory to several food spoilage bacteria and food-borne pathogens, including Bacillus cereus, Clostridium sporogenes, Enterococcus faecalis, Listeria spp. and Staphylococcus spp. Plantaricin 423 is resistant to treatment at 80 °C, but loses 50% of its activity after 60 min at 100 °C and 75% of its activity after autoclaving (121 °C, 15 min). Plantaricin 423 remains active after incubation at pH 1- 10 and is inactivated when treated with pepsin, papain, α-chymotrypsin, trypsin and Proteinase K. Plantaricin 423 was partially purified and its size estimated at 3.5 kDa, as determined by tricine-SDS-PAGE. The mechanism of activity of Plantaricin 423 is weakly bactericidal, as determined against Oenococcus oeni (previously Leuconostoc oenos). High DNA homology was obtained between the plasmid DNA of strain 423 and the pediocin PA-1 operon of Pediococcus acidilactici PAC 1.0, suggesting that plantaricin 423 is plasmid-encoded and related to the pediocin gene cluster.
dc.description.versionArticle
dc.identifier.citationJournal of Applied Microbiology
dc.identifier.citation84
dc.identifier.citation6
dc.identifier.issn13645072
dc.identifier.other10.1046/j.1365-2672.1998.00451.x
dc.identifier.urihttp://hdl.handle.net/10019.1/11170
dc.subjectbacteriocin
dc.subjectchymotrypsin a
dc.subjectdna
dc.subjectpapain
dc.subjectpepsin a
dc.subjectplantaricin
dc.subjectproteinase k
dc.subjecttrypsin
dc.subjectunclassified drug
dc.subjectarticle
dc.subjectautoclave
dc.subjectbactericidal activity
dc.subjectfood spoilage
dc.subjectgene cluster
dc.subjectlactobacillus plantarum
dc.subjectnonhuman
dc.subjectoperon
dc.subjectph
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectprotein isolation
dc.subjectprotein purification
dc.subjectsequence homology
dc.subjectsouthern blotting
dc.subjectBacteria
dc.subjectBacteriocins
dc.subjectBlotting, Southern
dc.subjectDNA, Bacterial
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectHeat
dc.subjectHydrogen-Ion Concentration
dc.subjectLactobacillus
dc.subjectMicrobial Sensitivity Tests
dc.subjectMutation
dc.subjectPeptide Hydrolases
dc.subjectPlasmids
dc.titleIsolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum
dc.typeArticle
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