Clonal expansion of a globally disseminated lineage of Mycobacterium tuberculosis with low IS6110 copy numbers

Simple item page
dc.contributor.authorWarren R.M.
dc.contributor.authorVictor T.C.
dc.contributor.authorStreicher E.M.
dc.contributor.authorRichardson M.
dc.contributor.authorVan Der Spuy G.D.
dc.contributor.authorJohnson R.
dc.contributor.authorChihota V.N.
dc.contributor.authorLocht C.
dc.contributor.authorSupply P.
dc.contributor.authorVan Helden P.D.
dc.date.accessioned2011-05-15T15:56:56Z
dc.date.available2011-05-15T15:56:56Z
dc.date.issued2004
dc.description.abstractKnowledge of the clonal expansion of Mycobacterium tuberculosis and accurate identification of predominant evolutionary lineages in this species remain limited, especially with regard to low-IS6110-copy-number strains. In this study, 170 M. tuberculosis isolates with ≤6 IS6110 insertions identified in Cape Town, South Africa, were characterized by principal genetic grouping, restriction fragment length polymorphism analysis, spoligotyping, IS6110 insertion site mapping, and variable-number tandem repeat (VNTR) typing. These analyses indicated that all but one of the isolates analyzed were members of principal genetic group 2 and of the same low-IS6110-copy-number lineage. The remaining isolate was a member of principal genetic group 1 and a different low-IS6110-copy-number lineage. Phylogenetic reconstruction suggests clonal expansion through sequential acquisition of additional IS6110 copies, expansion and contraction of VNTR sequences, and the deletion of specific direct-variable-repeat sequences. Furthermore, comparison of the genotypic data of 91 representative low-IS6110-copy-number isolates from Cape Town, other southern African regions, Europe, and the United States suggests that certain low-IS6110-copy-number strain spoligotypes and IS6110 fingerprints were acquired in the distant past. These clones have subsequently become widely disseminated and now play an important role in the global tuberculosis epidemic.
dc.description.versionArticle
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation42
dc.identifier.citation12
dc.identifier.issn951137
dc.identifier.other10.1128/JCM.42.12.5774-5782.2004
dc.identifier.urihttp://hdl.handle.net/10019.1/10122
dc.subjectarticle
dc.subjectbacterial genetics
dc.subjectbacterial genome
dc.subjectbacterial strain
dc.subjectclonal variation
dc.subjectEurope
dc.subjectevolutionary adaptation
dc.subjectgene deletion
dc.subjectgene insertion
dc.subjectgene mapping
dc.subjectgene number
dc.subjectgene sequence
dc.subjectgenetic analysis
dc.subjectgenetic epidemiology
dc.subjectgenetic variability
dc.subjectgenotype
dc.subjectmolecular cloning
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectphylogeny
dc.subjectpriority journal
dc.subjectrestriction fragment length polymorphism
dc.subjectsequential analysis
dc.subjectSouth Africa
dc.subjecttandem repeat
dc.subjectUnited States
dc.subjectDNA Transposable Elements
dc.subjectEurope
dc.subjectEvolution, Molecular
dc.subjectGene Dosage
dc.subjectHumans
dc.subjectMinisatellite Repeats
dc.subjectMycobacterium tuberculosis
dc.subjectOligonucleotides
dc.subjectPhylogeny
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Restriction Fragment Length
dc.subjectSouth Africa
dc.subjectTuberculosis
dc.subjectUnited States
dc.subjectActinobacteria (class)
dc.subjectBacteria (microorganisms)
dc.subjectinsertion sequences
dc.subjectMycobacterium
dc.subjectMycobacterium tuberculosis
dc.subjectuncultured actinomycete
dc.titleClonal expansion of a globally disseminated lineage of Mycobacterium tuberculosis with low IS6110 copy numbers
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications