Cloning and expression of the α-L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae

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dc.contributor.authorCrous J.M.
dc.contributor.authorPretorius I.S.
dc.contributor.authorVan Zyl W.H.
dc.date.accessioned2011-05-15T15:56:38Z
dc.date.available2011-05-15T15:56:38Z
dc.date.issued1996
dc.description.abstractFirst-strand cDNA was prepared from mRNA of Aspergillus niger MRC11624 induced on oat spelts xylan. Using the cDNA as a template, the α-L-arabinofuranosidase gene (abfB) was amplified with the polymerase chain reaction technique. The abfB DNA fragment was inserted between the yeast phosphoglycerate kinase I gene promoter (PGK1(P)) and terminator (PGK1(T)) sequences on a multicopy episomal plasmid. The resulting construct PGK1(P)abfB-PGK1(T) was designated ABF2. The ABF2 gene was expressed successfully in Saccharomyces cerevisiae and functional α-L-arabinofuranosidase was secreted from the yeast cells. The ABF2 nucleotide sequence was determined and verified to encode a 449-amino-acid protein (Abf2) that is 9.4% identical to the α-L-arabinofuranosidase B of A. niger N400. Maximum α-L-arabinofuranosidase activities of 0.020 U/ml and 1.40 U/ml were obtained with autoselective recombinant S. cerevisiae strains when grown for 48 h in synthetic and complex medium respectively.
dc.description.versionArticle
dc.identifier.citationApplied Microbiology and Biotechnology
dc.identifier.citation46
dc.identifier.citation3
dc.identifier.issn1757598
dc.identifier.other10.1007/s002530050813
dc.identifier.urihttp://hdl.handle.net/10019.1/9954
dc.subjectcomplementary dna
dc.subjectmessenger rna
dc.subjectarticle
dc.subjectaspergillus niger
dc.subjectcontrolled study
dc.subjectdna sequence
dc.subjectenzyme activity
dc.subjectfungus mutant
dc.subjectgene expression
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectsaccharomyces cerevisiae
dc.subjectAmino Acid Sequence
dc.subjectAspergillus niger
dc.subjectBase Sequence
dc.subjectBiotechnology
dc.subjectCloning, Molecular
dc.subjectDNA, Complementary
dc.subjectDNA, Fungal
dc.subjectGene Expression
dc.subjectGenes, Fungal
dc.subjectGlycoside Hydrolases
dc.subjectMolecular Sequence Data
dc.subjectSaccharomyces cerevisiae
dc.subjectSequence Homology, Amino Acid
dc.subjectAspergillus niger
dc.subjectFungi
dc.subjectSaccharomyces cerevisiae
dc.titleCloning and expression of the α-L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae
dc.typeArticle
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