Cloning and expression of the α-L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae
dc.contributor.author | Crous J.M. | |
dc.contributor.author | Pretorius I.S. | |
dc.contributor.author | Van Zyl W.H. | |
dc.date.accessioned | 2011-05-15T15:56:38Z | |
dc.date.available | 2011-05-15T15:56:38Z | |
dc.date.issued | 1996 | |
dc.description.abstract | First-strand cDNA was prepared from mRNA of Aspergillus niger MRC11624 induced on oat spelts xylan. Using the cDNA as a template, the α-L-arabinofuranosidase gene (abfB) was amplified with the polymerase chain reaction technique. The abfB DNA fragment was inserted between the yeast phosphoglycerate kinase I gene promoter (PGK1(P)) and terminator (PGK1(T)) sequences on a multicopy episomal plasmid. The resulting construct PGK1(P)abfB-PGK1(T) was designated ABF2. The ABF2 gene was expressed successfully in Saccharomyces cerevisiae and functional α-L-arabinofuranosidase was secreted from the yeast cells. The ABF2 nucleotide sequence was determined and verified to encode a 449-amino-acid protein (Abf2) that is 9.4% identical to the α-L-arabinofuranosidase B of A. niger N400. Maximum α-L-arabinofuranosidase activities of 0.020 U/ml and 1.40 U/ml were obtained with autoselective recombinant S. cerevisiae strains when grown for 48 h in synthetic and complex medium respectively. | |
dc.description.version | Article | |
dc.identifier.citation | Applied Microbiology and Biotechnology | |
dc.identifier.citation | 46 | |
dc.identifier.citation | 3 | |
dc.identifier.issn | 1757598 | |
dc.identifier.other | 10.1007/s002530050813 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/9954 | |
dc.subject | complementary dna | |
dc.subject | messenger rna | |
dc.subject | article | |
dc.subject | aspergillus niger | |
dc.subject | controlled study | |
dc.subject | dna sequence | |
dc.subject | enzyme activity | |
dc.subject | fungus mutant | |
dc.subject | gene expression | |
dc.subject | molecular cloning | |
dc.subject | nonhuman | |
dc.subject | saccharomyces cerevisiae | |
dc.subject | Amino Acid Sequence | |
dc.subject | Aspergillus niger | |
dc.subject | Base Sequence | |
dc.subject | Biotechnology | |
dc.subject | Cloning, Molecular | |
dc.subject | DNA, Complementary | |
dc.subject | DNA, Fungal | |
dc.subject | Gene Expression | |
dc.subject | Genes, Fungal | |
dc.subject | Glycoside Hydrolases | |
dc.subject | Molecular Sequence Data | |
dc.subject | Saccharomyces cerevisiae | |
dc.subject | Sequence Homology, Amino Acid | |
dc.subject | Aspergillus niger | |
dc.subject | Fungi | |
dc.subject | Saccharomyces cerevisiae | |
dc.title | Cloning and expression of the α-L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae | |
dc.type | Article |