Stability of Polymorphic GC-Rich Repeat Sequence-Containing Regions of Mycobacterium tuberculosis
Date
2004
Authors
Richardson M.
Van Der Spuy G.D.
Sampson S.L.
Beyers N.
Van Helden P.D.
Warren R.M.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Mycobacterium tuberculosis cultures were subjected to DNA fingerprinting with IS6110- and polymorphic GC-rich sequence (PGRS)-containing probes. The PGRS banding patterns remained highly stable during multiple cultures of specimens from one disease episode (0.5% changed) and during transmission in patients with close contact (1.9% changed). Characteristic PGRS-restriction fragment length polymorphism motifs for different strain groupings may indicate distant evolutionary events leading to the differentiation of M. tuberculosis strain lineages.
Description
Keywords
article, bacterial strain, bacterial transmission, bacterium culture, disease transmission, DNA fingerprinting, DNA probe, evolution, gene insertion sequence, Mycobacterium tuberculosis, nonhuman, nucleotide repeat, nucleotide sequence, pgrs restriction fragment length polymorphism motif, polymorphic guanine cytosine rich repeat sequence, priority journal, protein motif, protein stability, restriction fragment length polymorphism, strain difference, Cytosine, Dinucleotide Repeats, DNA Fingerprinting, DNA Transposable Elements, DNA, Bacterial, Evolution, Molecular, Genome, Bacterial, Guanine, Humans, Mycobacterium tuberculosis, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, Tuberculosis, Actinobacteria (class), Bacteria (microorganisms), insertion sequences, Mycobacterium, Mycobacterium tuberculosis, uncultured actinomycete, unidentified insertion sequence
Citation
Journal of Clinical Microbiology
42
3
42
3