Two gonadotropin-releasing hormone receptor subtypes with distinct ligand selectivity and differential distribution in brain and pituitary in the goldfish (Carassius auratus)

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dc.contributor.authorIlling N.
dc.contributor.authorTroskie B.E.
dc.contributor.authorNahorniak C.S.
dc.contributor.authorHapgood J.P.
dc.contributor.authorPeter R.E.
dc.contributor.authorMillar R.P.
dc.date.accessioned2011-05-15T16:02:21Z
dc.date.available2011-05-15T16:02:21Z
dc.date.issued1999
dc.description.abstractIn the goldfish (Carassius auratus) the two endogenous forms of gonadotropin-releasing hormone (GnRH), namely chicken GnRH II ([His5,Trp7,Tyr8]GnRH) and salmon GnRH ([Trp7,Leu8] GnRH), stimulate the release of both gonadotropins and growth hormone from the pituitary. This control is thought to occur by means of the stimulation of distinct GnRH receptors. These receptors can be distinguished on the basis of differential gonadotropin and growth hormone releasing activities of naturally occurring GnRHs and GnRHs with variant amino acids in position 8. We have cloned the cDNAs of two GnRH receptors, GfA and GfB, from goldfish brain and pituitary. Although the receptors share 71% identity, there are marked differences in their ligand selectivity. Both receptors are expressed in the pituitary but are differentially expressed in the brain, ovary, and liver. Thus we have found and cloned two full-length cDNAs that appear to correspond to different forms of GnRH receptor, with distinct pharmacological characteristics and tissue distribution, in a single species.
dc.description.versionArticle
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America
dc.identifier.citation96
dc.identifier.citation5
dc.identifier.issn278424
dc.identifier.other10.1073/pnas.96.5.2526
dc.identifier.urihttp://hdl.handle.net/10019.1/12424
dc.subjectcomplementary DNA
dc.subjectgonadorelin derivative
dc.subjectgonadorelin receptor
dc.subjectligand
dc.subjectreceptor subtype
dc.subjectanimal cell
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectgoldfish
dc.subjectgonadotropin release
dc.subjectgrowth hormone release
dc.subjecthypothalamus hypophysis system
dc.subjectmolecular cloning
dc.subjectmonkey
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpriority journal
dc.subjectreceptor density
dc.subjectsequence homology
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBrain
dc.subjectCloning, Molecular
dc.subjectDNA Primers
dc.subjectGoldfish
dc.subjectHumans
dc.subjectIn Situ Hybridization
dc.subjectMolecular Sequence Data
dc.subjectOrgan Specificity
dc.subjectPituitary Gland
dc.subjectPolymerase Chain Reaction
dc.subjectReceptors, LHRH
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectVariation (Genetics)
dc.subjectAnimalia
dc.subjectCarassius auratus
dc.subjectGallus gallus
dc.titleTwo gonadotropin-releasing hormone receptor subtypes with distinct ligand selectivity and differential distribution in brain and pituitary in the goldfish (Carassius auratus)
dc.typeArticle
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