Characterisation of non-viable whole barley, wheat and sorghum grains using near-infrared hyperspectral data and chemometrics
dc.contributor.author | McGoverin C.M. | |
dc.contributor.author | Engelbrecht P. | |
dc.contributor.author | Geladi P. | |
dc.contributor.author | Manley M. | |
dc.date.accessioned | 2011-10-13T16:58:27Z | |
dc.date.available | 2011-10-13T16:58:27Z | |
dc.date.issued | 2011 | |
dc.description.abstract | Undesired germination of cereal grains diminishes process utility and economic return. Pre-germination, the term used to describe untimely germination, leads to reduced viability of a grain sample. Accurate and rapid identification of non-viable grain is necessary to reduce losses associated with pre-germination. Viability of barley, wheat and sorghum grains was investigated with near-infrared hyperspectral imaging. Principal component analyses applied to cleaned hyperspectral images were able to differentiate between viable and non-viable classes in principal component (PC) five for barley and sorghum and in PC6 for wheat. An OH stretching and deformation combination mode (1,920-1,940 nm) featured in the loading line plots of these PCs; this water-based vibrational mode was a major contributor to the viable/non-viable differentiation. Viable and non-viable classes for partial least squares-discriminant analysis (PLS-DA) were assigned from PC scores that correlated with incubation time. The PLS-DA predictions of the viable proportion correlated well with the viable proportion observed using the tetrazolium test. Partial least squares regression analysis could not be used as a source of contrast in the hyperspectral images due to sampling issues. [Figure not available: see fulltext.] © 2011 Springer-Verlag. | |
dc.description.version | Article in Press | |
dc.identifier.citation | Analytical and Bioanalytical Chemistry | |
dc.identifier.citation | http://www.scopus.com/inward/record.url?eid=2-s2.0-80051481378&partnerID=40&md5=961d3a0fa982003e0319120cad961e69 | |
dc.identifier.issn | 16182642 | |
dc.identifier.other | 10.1007/s00216-011-5291-x | |
dc.identifier.uri | http://hdl.handle.net/10019.1/16729 | |
dc.subject | Barley | |
dc.subject | Near-infrared hyperspectral imaging | |
dc.subject | Pre-germination | |
dc.subject | Sorghum | |
dc.subject | Viability | |
dc.subject | Wheat | |
dc.title | Characterisation of non-viable whole barley, wheat and sorghum grains using near-infrared hyperspectral data and chemometrics | |
dc.type | Article in Press |