The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids

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dc.contributor.authorGrobler, J.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.contributor.authorSubden, R. E.en_ZA
dc.contributor.authorVan Vuren, H. J. J.en_ZA
dc.date.accessioned2011-05-15T16:05:35Z
dc.date.available2011-05-15T16:05:35Z
dc.date.issued1995
dc.description.abstractThe mae1 gene of the yeast Schizosaccharomyces pombe was identified on the basis of its ability to complement a mutant defective in the transport of malic acid. Analysis of the DNA sequence revealed an open reading frame of 1314 base pairs, encoding a polypeptide of 438 amino acids with a predicted molecular weight of 49 kDa. A hydropathy profile of the predicted amino acid sequence revealed a protein with ten membrane-spanning or associated domains and hydrophilic N- and C- termini. The predicted secondary structure of the protein is similar to models proposed for other integral membrane proteins from both prokaryotes and eukaryotes. The S. pombe mae1 gene encodes a single mRNA of 1.5 kb. The mae1 gene is expressed constitutively and is not subject to catabolite repression as was previously reported for the malate permease systems of Candida utilis and Hansenula anomala. The mae1 gene was mapped 2842 bp 5' to the MFm1 gene on chromosome I. Transport assays revealed that the mae1 gene encodes a permease involved in the uptake of L-malate, succinate and malonic acid. The sequence of the S. pombe mae1 gene is available in GenBank under Accession Number U21002.en_ZA
dc.description.versionArticle
dc.identifier.citationYeast
dc.identifier.citation11
dc.identifier.citation15
dc.identifier.issn0749503X
dc.identifier.other10.1002/yea.320111503
dc.identifier.urihttp://hdl.handle.net/10019.1/13199
dc.subjectdicarboxylic acid
dc.subjectmalic acid
dc.subjectmalonic acid
dc.subjectpermease
dc.subjectsuccinic acid
dc.subjectarticle
dc.subjectcell membrane permeability
dc.subjectdna sequence
dc.subjectdrug transport
dc.subjectenzyme structure
dc.subjectgene expression
dc.subjectgene mapping
dc.subjectgenetic analysis
dc.subjectpriority journal
dc.subjectschizosaccharomyces pombe
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectBiological Transport, Active
dc.subjectChromosomes, Fungal
dc.subjectCloning, Molecular
dc.subjectDicarboxylic Acids
dc.subjectDNA, Fungal
dc.subjectFermentation
dc.subjectGenes, Fungal
dc.subjectMalates
dc.subjectMembrane Transport Proteins
dc.subjectMolecular Sequence Data
dc.subjectOpen Reading Frames
dc.subjectProtein Structure, Secondary
dc.subjectRestriction Mapping
dc.subjectSchizosaccharomyces
dc.subjectSupport, Non-U.S. Gov't
dc.subjectCandida
dc.subjectEukaryota
dc.subjectPichia anomala
dc.subjectPichia jadinii
dc.subjectProkaryota
dc.subjectSchizosaccharomyces pombe
dc.titleThe mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids
dc.typeArticle
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