Epigenetic regulation of PGU1 transcription in Saccharomyces cerevisiae

Simple item page
dc.contributor.authorLouw, C.en_ZA
dc.contributor.authorYoung, P. R.en_ZA
dc.contributor.authorVan Rensburg, P.en_ZA
dc.contributor.authorDivol, Benoiten_ZA
dc.date.accessioned2011-05-15T16:03:26Z
dc.date.available2011-05-15T16:03:26Z
dc.date.issued2010
dc.description.abstractThe PGU1 gene of Saccharomyces cerevisiae has been shown to encode a polygalacturonase. The polygalacturonase activity in S. cerevisiae is strain specific. There are no significant differences in the PGU1 promoter regions of strains with and without polygalacturonase activity. The PGU1 gene is subtelomeric because it is located within 25 kb of the right telomere of chromosome X. Expressions of genes located in subtelomeric regions in the yeast S. cerevisiae are inhibited compared with the rest of the genome. In this study, we showed that the deletion of genes involved in telomere silencing enhances polygalacturonase activity. PGU1 transcription and polygalacturonase activity are increased when PGU1 is shifted to a different location in the genome, away from the telomere located close to this gene, and the depletion of the histone H4 leads to an increase in PGU1 transcription. We concluded that PGU1 is silenced in strains without polygalacturonase activity due to an epigenetic effect. The results of this study suggest that PGU1 is silenced by being folded into a heterochromatin-like structure at its subtelomeric position on chromosome X. Formation of this silent structure is dependent on the Isw2p chromatin remodeling complex, its histone fold motif containing subunit Dls1p and the N-terminal tail of the H4 histone. © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.
dc.description.versionArticle
dc.identifier.citationFEMS Yeast Research
dc.identifier.citation10
dc.identifier.citation2
dc.identifier.issn15671356
dc.identifier.other10.1111/j.1567-1364.2009.00599.x
dc.identifier.urihttp://hdl.handle.net/10019.1/12618
dc.subjectfungal protein
dc.subjecthistone
dc.subjectpolygalacturonase
dc.subjectprotein subunit
dc.subjectamino terminal sequence
dc.subjectarticle
dc.subjectchromatin assembly and disassembly
dc.subjectepigenetics
dc.subjectgene deletion
dc.subjectgene silencing
dc.subjectgenome
dc.subjectheterochromatin
dc.subjectnonhuman
dc.subjectprotein motif
dc.subjectSaccharomyces cerevisiae
dc.subjecttelomere
dc.subjectX chromosome
dc.subjectEpigenesis, Genetic
dc.subjectGene Deletion
dc.subjectGene Expression Regulation, Fungal
dc.subjectGene Silencing
dc.subjectHeterochromatin
dc.subjectHistones
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectPolygalacturonase
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectTelomere
dc.subjectSaccharomyces cerevisiae
dc.titleEpigenetic regulation of PGU1 transcription in Saccharomyces cerevisiae
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications