Carnitine and carnitine acetyltransferases in the yeast Saccharomyces cerevisiae: A role for carnitine in stress protection

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dc.contributor.authorFranken, J.en_ZA
dc.contributor.authorKroppenstedt, S.en_ZA
dc.contributor.authorSwiegers, J.H.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2011-05-15T16:01:28Z
dc.date.available2011-05-15T16:01:28Z
dc.date.issued2008
dc.description.abstractTo date, the only reported metabolic and physiological roles for carnitine in Saccharomyces cerevisiae are related to the activity of the carnitine shuttle. In yeast, the shuttle transfers peroxisomal activated acetyl-residues to the mitochondria. However, acetyl-CoA can also be metabolised by the glyoxylate cycle to form succinate. The two pathways, therefore, provide a metabolic bypass for each other, and carnitine-dependent phenotypes have only been described in strains with non-functional peroxisomal citrate synthase, Cit2p. Here, we present evidence for a role of carnitine in stress protection that is independent of CIT2 and of the carnitine shuttle. Data show that carnitine improves growth during oxidative stress and in the presence of weak organic acids in wt and in CAT deletion strains. Our data also show that strains with single, double and triple deletions of the three CAT genes generally present identical phenotypes, but that the deletion of CAT2 decreases survival during oxidative stress in a carnitine-independent manner. Overexpression of single CAT genes does not lead to cross-complementation, suggesting a highly specific metabolic role for each enzyme. The data suggest that carnitine protects cells from oxidative and organic acid stress, while CAT2 contributes to the response to oxidative stress. © Springer-Verlag 2008.
dc.description.versionArticle
dc.identifier.citationCurrent Genetics
dc.identifier.citation53
dc.identifier.citation6
dc.identifier.issn1728083
dc.identifier.other10.1007/s00294-008-0191-0
dc.identifier.urihttp://hdl.handle.net/10019.1/11994
dc.subjectacetyl coenzyme A
dc.subjectcarboxylic acid
dc.subjectcarnitine
dc.subjectcarnitine acetyltransferase
dc.subjectcitrate synthase
dc.subjectglyoxylic acid
dc.subjectsuccinic acid
dc.subjectarticle
dc.subjectcell growth
dc.subjectcell metabolism
dc.subjectcell protection
dc.subjectcell stress
dc.subjectcell survival
dc.subjectcontrolled study
dc.subjectenzyme metabolism
dc.subjectfluorescence microscopy
dc.subjectfungal strain
dc.subjectgene deletion
dc.subjectgene overexpression
dc.subjectgenetic complementation
dc.subjectmitochondrion
dc.subjectnonhuman
dc.subjectopen reading frame
dc.subjectoxidative stress
dc.subjectperoxisome
dc.subjectphenotype
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectprotein transport
dc.subjectSaccharomyces cerevisiae
dc.subjectCarbon
dc.subjectCarnitine
dc.subjectCarnitine O-Acetyltransferase
dc.subjectGene Deletion
dc.subjectModels, Biological
dc.subjectOxidative Stress
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae
dc.titleCarnitine and carnitine acetyltransferases in the yeast Saccharomyces cerevisiae: A role for carnitine in stress protection
dc.typeArticle
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