Development of a Listeria monocytogenes EGDe partial proteome reference map and comparison with the protein profiles of food isolates

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dc.contributor.authorRamnath M.
dc.contributor.authorRechinger K.B.
dc.contributor.authorJansch L.
dc.contributor.authorHastings J.W.
dc.contributor.authorKnochel S.
dc.contributor.authorGravesen A.
dc.date.accessioned2011-05-15T15:56:33Z
dc.date.available2011-05-15T15:56:33Z
dc.date.issued2003
dc.description.abstractA partially annotated proteome reference map of the food pathogen Listeria monocytogenes was developed for exponentially growing cells under standardized, optimal conditions by using the sequenced strain EGDe (serotype 1/2a) as a model organism. The map was developed by using a reproducible total protein extraction and two-dimensional (2-D) polyacrylamide gel electrophoresis analysis procedure, and it contained 33 identified proteins representing the four main protein functional classes. In order to facilitate analysis of membrane proteins, a protein compartmentalization procedure was assessed. The method used provided partial fractionation of membrane and cytosolic proteins. The total protein 2-D profiles of three serotype 1/2a strains and one serotype 1/2b strain isolated from food were compared to the L. monocytogenes EGDe proteome. An average of 13% of the major protein spots in the food strain proteomes were not matched in the strain EGDe proteome. The variation was greater for the less intense spots, and on average 28% of these spots were not matched. Two of the proteins identified in L. monocytogenes EGDe were missing in one or more of the food isolates. These two proteins were proteins involved in the main glycolytic pathway and in metabolism of coenzymes and prosthetic groups. The two corresponding genes were found by PCR amplification to be present in the four food isolates. Our results show that the L. monocytogenes EGDe reference map is a valuable starting point for analyses of strains having various origins and could be useful for analyzing the proteomes of different isolates of this pathogen.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation69
dc.identifier.citation6
dc.identifier.issn992240
dc.identifier.urihttp://hdl.handle.net/10019.1/9911
dc.subjectCells
dc.subjectFood products
dc.subjectProteins
dc.subjectProteomes
dc.subjectMicroorganisms
dc.subjectfood safety
dc.subjectmicrobiology
dc.subjectanimal cell
dc.subjectarticle
dc.subjectbacterial membrane
dc.subjectbacterium isolation
dc.subjectcell compartmentalization
dc.subjectfood analysis
dc.subjectfood industry
dc.subjectfood processing
dc.subjectgene sequence
dc.subjectglycolysis
dc.subjectinfection risk
dc.subjectisoelectric point
dc.subjectListeria monocytogenes
dc.subjectnonhuman
dc.subjectprotein analysis
dc.subjectproteomics
dc.subjectstrain difference
dc.subjectAnimals
dc.subjectBacterial Proteins
dc.subjectComputational Biology
dc.subjectElectrophoresis, Gel, Two-Dimensional
dc.subjectHumans
dc.subjectListeria monocytogenes
dc.subjectMeat
dc.subjectPeptide Mapping
dc.subjectProteome
dc.subjectReference Standards
dc.subjectSerotyping
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.subjectListeria
dc.subjectListeria monocytogenes
dc.subjectNegibacteria
dc.titleDevelopment of a Listeria monocytogenes EGDe partial proteome reference map and comparison with the protein profiles of food isolates
dc.typeArticle
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