Recovery of endo-polygalacturonase activity in wine yeast and its effect on wine aroma

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dc.contributor.authorVan Wyk, H.en_ZA
dc.contributor.authorDivol, Benoiten_ZA
dc.date.accessioned2011-05-15T16:03:26Z
dc.date.available2011-05-15T16:03:26Z
dc.date.issued2010
dc.description.abstractThe PGU1 gene, which encodes an endo-polygalacturonase, is present in most strains of Saccharomyces cerevisiae. In some strains that lack activity, the PGU1 gene was replaced by a partial transposon. In an attempt to restore enzyme activity, the PGU1 gene was successfully reintroduced into its native position in five of these strains. Polygalacturonase activity was recovered in strains UCLMS-1, -3 and -4, while Collection Cépage Cabernet and L2226 did not show any transcription of the gene after integration. Small-scale fermentations, under conditions mimicking those of winemaking, were carried out with the recombinant strains to determine the effect of an endo-polygalacturonase- producing wine strain on the wine aroma. Some statistically significant tendencies were observed for the production of certain compounds whose concentrations were higher in the wine made from the recombinant strains than that from their respective wild types. These differences might therefore induce a slight change in the aroma profile of the wines. Moreover, recovery of endo-polygalacturonase activity in certain wine strains did, however, provide an insight into the regulation of PGU1 in these strains. © 2009 Federation of European Microbiological Societies.
dc.description.versionArticle
dc.identifier.citationFEMS Yeast Research
dc.identifier.citation10
dc.identifier.citation1
dc.identifier.issn15671356
dc.identifier.other10.1111/j.1567-1364.2009.00588.x
dc.identifier.urihttp://hdl.handle.net/10019.1/12619
dc.subjectpolygalacturonase
dc.subjectaroma
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectenzyme activity
dc.subjectfermentation
dc.subjectfungal gene
dc.subjectfungal strain
dc.subjectgenetic transcription
dc.subjectnonhuman
dc.subjectPGU1 gene
dc.subjectregulatory mechanism
dc.subjectSaccharomyces cerevisiae
dc.subjecttransposon
dc.subjectwild type
dc.subjectwine
dc.subjectyeast
dc.subjectGene Expression Regulation, Fungal
dc.subjectGenetic Engineering
dc.subjectIndustrial Microbiology
dc.subjectMetabolic Networks and Pathways
dc.subjectPolygalacturonase
dc.subjectRecombination, Genetic
dc.subjectSaccharomyces cerevisiae
dc.subjectWine
dc.subjectSaccharomyces cerevisiae
dc.titleRecovery of endo-polygalacturonase activity in wine yeast and its effect on wine aroma
dc.typeArticle
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