A rapid high-performance liquid chromatography (HPLC) method for the extraction and quantification of folates in dairy products and cultures of Propionibacterium freudenreichii

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dc.contributor.authorvan Wyk J.
dc.contributor.authorBritz T.J.
dc.date.accessioned2012-04-12T08:18:57Z
dc.date.available2012-04-12T08:18:57Z
dc.date.issued2012
dc.description.abstractNutritional folate deficiencies in Southern African communities necessitated mandatory fortification. Current microbiological assays (MA) used to measure food folates, essential for quality control and regulatory purposes, are time-consuming. This study describes an alternative extraction and detection method for folates in dairy products and Propionibacterium freudenreichii cultures. Folates were extracted by heating with a phosphate buffer (pH 6.0). Polyglutamates were deconjugated with chicken pancreas and hog kidney deconjugases. Samples were purified using strong anion exchange solid phase extraction. Reversed-phase high-performance liquid chromatography (HPLC) using an acetonitrile-phosphate buffer (pH 2.2) gradient effectively separated four vitamers. Fluorescence (tetrahydrofolate (THF), 5-CH 3-THF and 5-CHO-THF), and UV detection (folic acid) were used, calibration curves were linear (R 2>0.0997), and detection and quantification limits were 0.0006 to 0.015 and 0.002 to 0.05 μg/ml, respectively. Accuracy was 80 to 108% and intra- and inter-day precision [%relative standard deviation (%RSD)] were lower than 4%. The method, validated against the standard MA, is a selective, sensitive, reliable and rapid alternative. © 2012 Academic Journals.
dc.identifier.citationAfrican Journal of Biotechnology
dc.identifier.citation11
dc.identifier.citation8
dc.identifier.citation2087
dc.identifier.citation2098
dc.identifier.issn16845315
dc.identifier.other10.5897/AJB11.2491
dc.identifier.urihttp://hdl.handle.net/10019.1/20515
dc.subjectFolate
dc.subjectHigh-performance liquid chromatography (HPLC)
dc.subjectMicrobiological folate assay
dc.subjectPropionibacterium freudenreichii
dc.titleA rapid high-performance liquid chromatography (HPLC) method for the extraction and quantification of folates in dairy products and cultures of Propionibacterium freudenreichii
dc.typeArticle
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