Immunosuppression during active tuberculosis is characterized by decreased interferon-γ production and CD25 expression with elevated forkhead box P3, transforming growth factor-β, and interleukin-4 mRNA levels

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dc.contributor.authorRoberts T.
dc.contributor.authorBeyers N.
dc.contributor.authorAguirre A.
dc.contributor.authorWalzl G.
dc.date.accessioned2011-05-15T16:03:33Z
dc.date.available2011-05-15T16:03:33Z
dc.date.issued2007
dc.description.abstractThe balance between effector and regulatory responses after Mycobacterium tuberculosis infection may dictate outcome and progression to active disease. We investigated effector and regulatory T cell responses in bacille Calmette-Guérin (BCG)-stimulated peripheral blood mononuclear cells and whole blood cultures from persons with active tuberculosis (TB), persons with TB at the end of 6 months of treatment, and healthy control subjects with latent TB infection. All 3 groups displayed BCG-induced increases in effector and regulatory T cell phenotypes as defined by CD4+CD25lo and CD4+CD25hi T cells, respectively. In case patients with active disease, BCG stimulation induced the lowest increase of CD25, CD4 +CD25hi, CTLA-4, and interferon-γ. However, these case patients expressed the highest mRNA levels of forkhead box P3, transforming growth factor (TGF)-β, and interleukin (IL)-4 and a lower T-bet:GATA-3 ratio. There were no significant differences in IL-4δ2, IL-10, or TGF-β receptor-II mRNA expression between groups. Together, these results suggest that immunosuppression seen after mycobacterial stimulation in case patients with active TB is associated with naturally occurring regulatory T cells. © 2007 by the Infectious Diseases Society of America. All rights reserved.
dc.description.versionArticle
dc.identifier.citationJournal of Infectious Diseases
dc.identifier.citation195
dc.identifier.citation6
dc.identifier.issn221899
dc.identifier.other10.1086/511277
dc.identifier.urihttp://hdl.handle.net/10019.1/12676
dc.subjectBCG vaccine
dc.subjectethambutol
dc.subjectgamma interferon
dc.subjectinterleukin 10
dc.subjectinterleukin 2 receptor alpha
dc.subjectinterleukin 4
dc.subjectisoniazid
dc.subjectmessenger RNA
dc.subjectpyrazinamide
dc.subjectrifampicin
dc.subjecttranscription factor FOXP3
dc.subjecttranscription factor GATA 3
dc.subjecttranscription factor T bet
dc.subjecttransforming growth factor beta
dc.subjecttransforming growth factor beta receptor 2
dc.subjectantigen expression
dc.subjectarticle
dc.subjectCD4+ CD25+ T lymphocyte
dc.subjectcellular immunity
dc.subjectcontrolled study
dc.subjecteffector cell
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmune deficiency
dc.subjectinterferon production
dc.subjectlung tuberculosis
dc.subjectmononuclear cell
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectpriority journal
dc.subjectregulatory T lymphocyte
dc.subjectAntigens, CD
dc.subjectAntigens, CD3
dc.subjectBCG Vaccine
dc.subjectCD4 Lymphocyte Count
dc.subjectDNA Primers
dc.subjectForkhead Transcription Factors
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectImmunosuppression
dc.subjectInterferon Type II
dc.subjectInterleukin-2 Receptor alpha Subunit
dc.subjectInterleukin-4
dc.subjectLymphocyte Count
dc.subjectMycobacterium tuberculosis
dc.subjectPolymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectT-Lymphocytes
dc.subjectTransforming Growth Factor beta
dc.subjectTuberculosis
dc.titleImmunosuppression during active tuberculosis is characterized by decreased interferon-γ production and CD25 expression with elevated forkhead box P3, transforming growth factor-β, and interleukin-4 mRNA levels
dc.typeArticle
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