Development of on-line high performance liquid chromatography (HPLC)-Biochemical detection methods as tools in the identification of bioactives
dc.contributor.author | Malherbe C.J. | |
dc.contributor.author | de Beer D. | |
dc.contributor.author | Joubert E. | |
dc.date.accessioned | 2012-04-12T08:33:55Z | |
dc.date.available | 2012-04-12T08:33:55Z | |
dc.date.issued | 2012 | |
dc.description.abstract | Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays. © 2012. | |
dc.identifier.citation | International Journal of Molecular Sciences | |
dc.identifier.citation | 13 | |
dc.identifier.citation | 3 | |
dc.identifier.citation | 3101 | |
dc.identifier.citation | 3133 | |
dc.identifier.issn | 14220067 | |
dc.identifier.other | 3101-3133 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/20735 | |
dc.subject | Antioxidant | |
dc.subject | Bioactive phytochemicals | |
dc.subject | Biochemical detection | |
dc.subject | Enzyme inhibition | |
dc.subject | HPLC | |
dc.subject | Receptor affinity | |
dc.title | Development of on-line high performance liquid chromatography (HPLC)-Biochemical detection methods as tools in the identification of bioactives | |
dc.type | Review |