Taxonomic re-evaluation of Leptographium lundbergii based on DNA sequence comparisons and morphology

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dc.contributor.authorJacobs K.
dc.contributor.authorSolheim H.
dc.contributor.authorWingfield B.D.
dc.contributor.authorWingfield M.J.
dc.date.accessioned2011-05-15T15:57:24Z
dc.date.available2011-05-15T15:57:24Z
dc.date.issued2005
dc.description.abstractThe genus Leptographium was described in 1927 and currently includes 48 species, with L. lundbergii as the type species. In recent years, the taxonomic status of L. lundbergii has not been uniformly agreed upon and it has been the topic of considerable debate. The problem was compounded by the absence of a type specimen, and the species was epitypified at a later stage. Unfortunately, the whereabouts of the epitype is now unknown. In 1983, Wingfield & Marasas described L. truncatum, which is morphologically similar to L. lundbergii. Based on DNA comparisons and similarities in their morphology, this fungus was reduced to synonymy with L. lundbergii. The loss of the type specimen as well as variation in the morphology of strains identified as L. lundbergii prompted us to re-examine the taxonomic status of this species. A number of strains from various geographic areas were studied. These include a strain of L. lundbergii deposited at CBS by Melin in 1929 (CBS 352.29) as well as the ex-type strain of L. truncatum. The strains were compared based on morphology and comparison of multiple gene sequences. Three genes or genic regions, ITS2 and part of the 28S gene, partial β-tubulin and partial elongation factor 1-α were compared. Strains currently identified as L. lundbergii, represented a complex of species. Strains initially described as L. truncatum clustered separately from other L. lundbergii strains, could be distinguished morphologically and should be treated as a distinct taxon. L. lundbergii is provided with a new and expanded description based on a neotype designated for it. A third group was also identified as separate from the main L. lundbergii clade and had a distinct Hyalorhinocladiella-type anamorph, described here as H. pinicola sp. nov. © The British Mycological Society.
dc.description.versionArticle
dc.identifier.citationMycological Research
dc.identifier.citation109
dc.identifier.citation10
dc.identifier.issn9537562
dc.identifier.other10.1017/S0953756205003618
dc.identifier.urihttp://hdl.handle.net/10019.1/10378
dc.subjectfungal DNA
dc.subjectribosomal spacer DNA
dc.subjectribosome RNA
dc.subjecttubulin
dc.subjectfungus
dc.subjectgenetic analysis
dc.subjectmorphology
dc.subjecttaxonomy
dc.subjectarticle
dc.subjectAscomycetes
dc.subjectchemistry
dc.subjectclassification
dc.subjectDNA sequence
dc.subjectgenetics
dc.subjectnucleotide sequence
dc.subjectphase contrast microscopy
dc.subjectphylogeny
dc.subjectpolymerase chain reaction
dc.subjectsequence alignment
dc.subjectultrastructure
dc.subjectAscomycota
dc.subjectBase Sequence
dc.subjectDNA, Fungal
dc.subjectDNA, Ribosomal Spacer
dc.subjectMicroscopy, Interference
dc.subjectMicroscopy, Phase-Contrast
dc.subjectPhylogeny
dc.subjectPolymerase Chain Reaction
dc.subjectRNA, Ribosomal
dc.subjectSequence Alignment
dc.subjectSequence Analysis, DNA
dc.subjectTubulin
dc.subjectFungi
dc.subjectHyalorhinocladiella
dc.subjectLeptographium
dc.subjectLeptographium lundbergii
dc.titleTaxonomic re-evaluation of Leptographium lundbergii based on DNA sequence comparisons and morphology
dc.typeArticle
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