Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium

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dc.contributor.authorDonninger H.
dc.contributor.authorGlashoff R.
dc.contributor.authorHaitchi H.-M.
dc.contributor.authorSyce J.A.
dc.contributor.authorGhildyal R.
dc.contributor.authorVan Rensburg E.
dc.contributor.authorBardin P.G.
dc.date.accessioned2011-05-15T16:03:34Z
dc.date.available2011-05-15T16:03:34Z
dc.date.issued2003
dc.description.abstractEpithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.
dc.description.versionArticle
dc.identifier.citationJournal of Infectious Diseases
dc.identifier.citation187
dc.identifier.citation11
dc.identifier.issn221899
dc.identifier.other10.1086/375246
dc.identifier.urihttp://hdl.handle.net/10019.1/12679
dc.subjectalpha chemokine
dc.subjectepithelial derived neutrophil activating factor 78
dc.subjectinterleukin 8
dc.subjectmessenger RNA
dc.subjectarticle
dc.subjectasthma
dc.subjectbronchus mucosa
dc.subjectcell activation
dc.subjectcell line
dc.subjectcontrolled study
dc.subjectdisease exacerbation
dc.subjectenzyme linked immunosorbent assay
dc.subjectepithelium cell
dc.subjectgene construct
dc.subjectgenetic transcription
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectnonhuman
dc.subjectnucleic acid analysis
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectprotein function
dc.subjectprotein induction
dc.subjectprotein secretion
dc.subjectquantitative analysis
dc.subjectreverse transcription polymerase chain reaction
dc.subjectRhinovirus
dc.subjectvirus induction
dc.subjectvirus strain
dc.subjectAdult
dc.subjectAsthma
dc.subjectBronchi
dc.subjectCell Line
dc.subjectCells, Cultured
dc.subjectChemokines, CXC
dc.subjectEpithelium
dc.subjectFemale
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectInterleukin-8
dc.subjectMale
dc.subjectPicornaviridae Infections
dc.subjectPromoter Regions (Genetics)
dc.subjectRhinovirus
dc.subjectRNA, Messenger
dc.subjectTime Factors
dc.subjectTranscription, Genetic
dc.subjectVirus Replication
dc.titleRhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium
dc.typeArticle
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