MSS11, a novel yeast gene involved in the regulation of starch metabolism

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dc.contributor.authorWebber A.L.
dc.contributor.authorLambrechts M.G.
dc.contributor.authorPretorius I.S.
dc.date.accessioned2011-05-15T16:01:29Z
dc.date.available2011-05-15T16:01:29Z
dc.date.issued1997
dc.description.abstractExpression of the STA1-3 glucoamylase genes, responsible for starch degradation in Saccharomyces cerevisiae, is down regulated by the presence of STA10. In order to elucidate the role of STA10 in the regulation of the glucoamylase system, a multicopy genomic library was constructed and screened for genes that enhanced growth of a STA2-STA10 S. cerevisiae strain on starch media. This screen allowed us to clone and characterize a novel activator gene of STA2 (and by extrapolation, STA1 and STA3), designated MSS11. A strain transformed with multiple copies of MSS11 exhibits increased levels of STA2 mRNA and, consequently, increased glucoamylase activity. Deletion of MSS11, located on chromosome XIII, results in media-dependent absence of glucoamylase synthesis. MSS11 has not been cloned previously and the encoded protein, Mss11p, is not homologous to any other known protein. An outstanding feature of Mss11p is that the protein contains regions of 33 asparagine residues interrupted by only three serine residues, and 35 glutamine residues interrupted by a single histidine residue. Epistasis studies showed that deletion of MSS11 abolishes the activation of STA2 caused by the over-expression of MSS10, a previously identified gene. In turn, it was found that deletion of MSS10 still allows activation of STA2 by over-expression of MSS11. Mss11p therefore appears to be positioned below Mss10p in a signal transduction pathway.
dc.description.versionArticle
dc.identifier.citationCurrent Genetics
dc.identifier.citation32
dc.identifier.citation4
dc.identifier.issn1728083
dc.identifier.other10.1007/s002940050275
dc.identifier.urihttp://hdl.handle.net/10019.1/12003
dc.subjectglucan 1,4 alpha glucosidase
dc.subjecthistidine
dc.subjectmessenger rna
dc.subjectstarch
dc.subjectarticle
dc.subjectcarbohydrate metabolism
dc.subjectchromosome
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectsaccharomyces cerevisiae
dc.subjectsignal transduction
dc.subjectAmino Acid Sequence
dc.subjectChromosome Mapping
dc.subjectChromosomes, Fungal
dc.subjectCloning, Molecular
dc.subjectEscherichia coli
dc.subjectFungal Proteins
dc.subjectGene Deletion
dc.subjectGene Dosage
dc.subjectGene Expression Regulation, Fungal
dc.subjectGenotype
dc.subjectGlucan 1,4-alpha-Glucosidase
dc.subjectMolecular Sequence Data
dc.subjectPlasmids
dc.subjectRecombinant Proteins
dc.subjectRestriction Mapping
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectSignal Transduction
dc.subjectStarch
dc.subjectSaccharomyces cerevisiae
dc.titleMSS11, a novel yeast gene involved in the regulation of starch metabolism
dc.typeArticle
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