An ffh mutant of Streptococcus mutans is viable and able to physiologically adapt to low pH in continuous culture
dc.contributor.author | Crowley P.J. | |
dc.contributor.author | Svensater G. | |
dc.contributor.author | Snoep J.L. | |
dc.contributor.author | Bleiweis A.S. | |
dc.contributor.author | Brady L.J. | |
dc.date.accessioned | 2011-05-15T16:04:57Z | |
dc.date.available | 2011-05-15T16:04:57Z | |
dc.date.issued | 2004 | |
dc.description.abstract | Previously, we described in Streptococcus mutans strain NG8 a 5-gene operon (sat) that includes ffh, the bacterial homologue of the eukaryotic signal recognition particle (SRP) protein, SR54. A mutation in ffh resulted in acid sensitivity but not loss of viability. In the present study, chemostat-grown cells of the ffh mutant were shown to possess only 26% and 39% of the parental membrane F-ATPase activity and 55% and 75% of parental glucose- phosphotransferase (PTS) activity when pH-7 and pH-5-grown cells, respectively, were assayed. Two-dimensional-gel electrophoretic analyses revealed significant differences in protein profiles between parent and ffh-mutant strains at both pH 5 and pH 7. It appears that the loss of active SRP (Ffh) function, while not lethal, results in substantial alterations in cellular physiology that includes acid tolerance. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. | |
dc.description.version | Article | |
dc.identifier.citation | FEMS Microbiology Letters | |
dc.identifier.citation | 234 | |
dc.identifier.citation | 2 | |
dc.identifier.issn | 3781097 | |
dc.identifier.other | 10.1016/j.femsle.2004.03.043 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/12899 | |
dc.subject | adenosine triphosphatase | |
dc.subject | glucokinase | |
dc.subject | signal recognition particle | |
dc.subject | acid tolerance | |
dc.subject | acidity | |
dc.subject | alkalinity | |
dc.subject | article | |
dc.subject | bacterial cell | |
dc.subject | bacterial gene | |
dc.subject | bacterial growth | |
dc.subject | bacterial survival | |
dc.subject | bacterium culture | |
dc.subject | bacterium mutant | |
dc.subject | cell viability | |
dc.subject | chemostat | |
dc.subject | continuous culture | |
dc.subject | controlled study | |
dc.subject | enzyme activity | |
dc.subject | nonhuman | |
dc.subject | priority journal | |
dc.subject | protein function | |
dc.subject | Streptococcus mutans | |
dc.subject | two dimensional gel electrophoresis | |
dc.subject | Acclimatization | |
dc.subject | Bacterial Proteins | |
dc.subject | Cell Membrane | |
dc.subject | Hydrogen-Ion Concentration | |
dc.subject | Kinetics | |
dc.subject | Operon | |
dc.subject | Phosphoenolpyruvate Sugar Phosphotransferase System | |
dc.subject | Proton-Translocating ATPases | |
dc.subject | Signal Recognition Particle | |
dc.subject | Streptococcus mutans | |
dc.subject | Substrate Specificity | |
dc.subject | Bacteria (microorganisms) | |
dc.subject | Eukaryota | |
dc.subject | Streptococcus | |
dc.subject | Streptococcus mutans | |
dc.title | An ffh mutant of Streptococcus mutans is viable and able to physiologically adapt to low pH in continuous culture | |
dc.type | Article |