A downstream process for production of a viable and stable Bacillus cereus aquaculture biological agent
Date
2010
Authors
Lalloo R.
Maharajh D.
Gorgens J.
Gardiner N.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Biological products offer advantages over chemotherapeutics in aquaculture. Adoption in commercial application is lacking due to limitations in process and product development that address key end user product requirements such as cost, efficacy, shelf life and convenience. In previous studies, we have reported on the efficacy, physiological robustness and low-cost spore production of a Bacillus cereus isolate (NRRL 100132). This study examines the development of suitable spore recovery, drying, formulation and tablet production from the fermentation product. Key criteria used for such downstream process unit evaluation included spore viability, recovery, spore balance, spore re-germination, product intermediate stability, end product stability and efficacy. A process flow sheet comprising vertical tube centrifugation, fluidised bed agglomeration and tablet pressing yielded a suitable product. The formulation included corn steep liquor and glucose to enhance subsequent spore re-germination. Viable spore recovery and spore balance closure across each of the process units was high (>70% and >99% respectively), with improvement in recovery possible by adoption of continuous processing at large scale. Spore re-germination was 97%, whilst a product half-life in excess of 5 years was estimated based on thermal resistance curves. The process resulted in a commercially attractive product and suitable variable cost of production. © 2009 Springer-Verlag.
Description
Keywords
Biological agents, Biological Products, Commercial applications, Continuous processing, Corn steep liquor, Downstream process, Downstream-processing, End users, End-products, Fermentation products, Fluidised bed agglomeration, In-process, Process flow-sheet, Process unit, Product requirements, Shelf life, Thermal resistance, Variable costs, Vertical tube, Agglomeration, Agriculture, Aquaculture, Bacteriology, Centrifugation, Cost accounting, Fluidization, Fluidized beds, Glucose, Product development, Stability criteria, Bacillus cereus, glucose, aquaculture, bacterium, chemotherapy, germination, physiological response, sporulation, aquaculture, article, Bacillus cereus, bacterial spore, bacterial viability, biological control agent, continuous process, cost benefit analysis, fluidized bed reactor, liquid, nonhuman, spore germination, Aquaculture, Bacillus cereus, Biological Products, Culture Media, Fermentation, Freeze Drying, Glucose, Microbial Viability, Pest Control, Biological, Spores, Bacterial, Zea mays, Bacillus cereus, Polygala incarnata, Zea mays
Citation
Applied Microbiology and Biotechnology
86
2
86
2