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Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility

Streicher, Elizabeth M. ; Maharaj, Kashmeel ; York, Talita ; Van Heerden, Carel ; Barnard, Marinus ; Diacon, Andreas ; Mendel, Carl M. ; Bosman, Marlein E. ; Hepple, Juli A. ; Pym, Alexander S. ; Warren, Robin M. ; Van Helden, Paul D. (2014)

CITATION: Streicher, E. M., et al. 2014. Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility. Journal of Clinical Microbiology, 52(11): 4056-4057. doi: 10.1128/JCM.02438-14.

The original publication is available at http://jcm.asm.org

Article

We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susceptibility to pyrazinamide.

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/99042
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