Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility

Streicher, Elizabeth M. ; Maharaj, Kashmeel ; York, Talita ; Van Heerden, Carel ; Barnard, Marinus ; Diacon, Andreas ; Mendel, Carl M. ; Bosman, Marlein E. ; Hepple, Juli A. ; Pym, Alexander S. ; Warren, Robin M. ; Van Helden, Paul D. (2014)

CITATION: Streicher, E. M., et al. 2014. Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility. Journal of Clinical Microbiology, 52(11): 4056-4057. doi: 10.1128/JCM.02438-14.

The original publication is available at http://jcm.asm.org

Article

We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susceptibility to pyrazinamide.

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