Analysis of vitamin B6 vitamers in plasma by cation-exchange high-performance liquid chromatography
Date
1987
Authors
Shephard G.S.
Louw M.E.J.
Labadarios D.
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Abstract
The aims of the present study were to evaluate the cation-exchange HPLC assay published by Coburn and Mahuren using 4'-deoxypyridoxine 5'-phosphate as an internal standard, to compare the accuracy of this method of pyridoxal 5'-phosphate (PLP) analysis with the established tyrosine decarboxylase apo-enzyme method and to report on various problems encountered during the development of this assay in our laboratory, in particular the importance of the method of blood collection and the effects of storage of standard mixtures of the vitamin B6 vitamers.
Description
Keywords
deoxypyridoxine phosphate, pyridoxal, pyridoxal 5 phosphate, pyridoxamine, pyridoxic acid, pyridoxine, unclassified drug, blood and hemopoietic system, drug analysis, drug blood level, drug determination, drug stability, etiology, high performance liquid chromatography, human, human cell, methodology, normal value, plasma, preliminary communication, preschool child, priority journal, school child, vitamer, article, blood, ion exchange chromatography, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Human, Pyridoxine
Citation
Journal of Chromatography - Biomedical Applications
416
1
416
1