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Analysis of vitamin B6 vitamers in plasma by cation-exchange high-performance liquid chromatography

dc.contributor.authorShephard G.S.
dc.contributor.authorLouw M.E.J.
dc.contributor.authorLabadarios D.
dc.date.accessioned2011-05-15T16:16:54Z
dc.date.available2011-05-15T16:16:54Z
dc.date.issued1987
dc.identifier.citationJournal of Chromatography - Biomedical Applications
dc.identifier.citation416
dc.identifier.citation1
dc.identifier.urihttp://hdl.handle.net/10019.1/13985
dc.description.abstractThe aims of the present study were to evaluate the cation-exchange HPLC assay published by Coburn and Mahuren using 4'-deoxypyridoxine 5'-phosphate as an internal standard, to compare the accuracy of this method of pyridoxal 5'-phosphate (PLP) analysis with the established tyrosine decarboxylase apo-enzyme method and to report on various problems encountered during the development of this assay in our laboratory, in particular the importance of the method of blood collection and the effects of storage of standard mixtures of the vitamin B6 vitamers.
dc.subjectdeoxypyridoxine phosphate
dc.subjectpyridoxal
dc.subjectpyridoxal 5 phosphate
dc.subjectpyridoxamine
dc.subjectpyridoxic acid
dc.subjectpyridoxine
dc.subjectunclassified drug
dc.subjectblood and hemopoietic system
dc.subjectdrug analysis
dc.subjectdrug blood level
dc.subjectdrug determination
dc.subjectdrug stability
dc.subjectetiology
dc.subjecthigh performance liquid chromatography
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmethodology
dc.subjectnormal value
dc.subjectplasma
dc.subjectpreliminary communication
dc.subjectpreschool child
dc.subjectpriority journal
dc.subjectschool child
dc.subjectvitamer
dc.subjectarticle
dc.subjectblood
dc.subjection exchange chromatography
dc.subjectChromatography, High Pressure Liquid
dc.subjectChromatography, Ion Exchange
dc.subjectHuman
dc.subjectPyridoxine
dc.titleAnalysis of vitamin B6 vitamers in plasma by cation-exchange high-performance liquid chromatography


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