Effect of medroxyprogesterone acetate contraception on cytoplasmic estrogen receptor content of the human cervix uteri
Date
1978
Authors
Rall H.J.S.
Soto Ferreira G.
Janssens K.Y.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Specimens of cervical tissue from 5 women each on 150 mg and 450 mg regimens of medroxyprogesterone acetate (MPA) for contraceptive purposes, were obtained through punch biopsy 15 days after injection. In another group of 5 women each on the same contraceptive regimens, punch biopsies of the cervix uteri were obtained 30 days after injection. These times corresponded to maximum and optimum blood levels of MPA respectively. Corresponding tissue from the same anatomical position in patients matched, where possible, for age and parity was obtained from hysterectomy specimens to serve as controls. Quantification of estrogen receptor content in the cytoplasm of these tissues was achieved through standard procedures. Analysis of data showed that MPA suppressed estrogen receptor content significantly compared to controls, but that there were no differences in this effect between the 2 dosages or time of biopsy.The effect of medroxyprogesterone acetate (MPA) contraception on cytoplasmic estrogen receptor content of the human cervix uteri was investigated. Cervical specimens from 5 women each on 150 mg and 450 mg regimens of MPA were obtained through punch biopsy 15 days postinjection. In another similar group of 5 women, specimens were obtained 30 days postinjection. These times corresponded to maximum and optimum blood levels of MPA, respectively. Corresponding tissue from hysterectomy specimens served as controls. Quantification of estrogen receptor content in the cytoplasm was achieved through standard procedures. There was a highly significant difference (p .000001) between the MPA treated and control groups; the mean femtomole values were 120.6 and 271.39, respectively. These results are indicative of drastic suppression of estrogen receptors by MPA. No differences between the 2 dosage regimens or time of biopsy were found in regard to this effect.
Description
Keywords
drug receptor, estrogen receptor, medroxyprogesterone acetate, animal experiment, contraception, cytology, dose response, drug response, estrogen therapy, female genital system, histology, intramuscular drug administration, parenteral drug administration, pharmacokinetics, uterine cervix, Age Factors, Biology, Cervical Effects, Cervix, Clinical Research, Contraception, Contraceptive Agents, Female--administraction and dosage, Contraceptive Agents, Female--side effects, Contraceptive Agents, Progestin--administraction and dosage, Contraceptive Agents, Progestin--side effects, Contraceptive Agents--administraction and dosage, Contraceptive Agents--side effects, Demographic Factors, Examinations And Diagnoses, Family Planning, Fertility, Fertility Measurements, Genitalia, Genitalia, Female, Hormone Receptors, Injectables, Laboratory Examinations And Diagnoses, Laboratory Procedures, Medroxyprogesterone Acetate--administraction and dosage, Medroxyprogesterone Acetate--side effects, Membrane Proteins, Parity, Physiology, Population, Population Characteristics, Population Dynamics, Research Methodology, Urogenital System, Uterus, Women, Adult, Cervix Uteri, Comparative Study, Cytoplasm, Female, Human, Hysterectomy, Medroxyprogesterone, Middle Age, Parity, Pregnancy, Receptors, Estrogen, Time Factors
Citation
International Journal of Fertility
23
1
23
1